PMID: 8940663Aug 1, 1996Paper

Acrosome reaction stimulated by the GTP non-hydrolizable analogue GTP gamma S is blocked by phospholipase A2 inhibitors in human spermatozoa

International Journal of Andrology
L DomínguezL S Mayorga

Abstract

Phospholipase A2 (PLA2, EC 3.1.1.4) is involved in the cascade of signalling events leading to the acrosome reaction in human spermatozoa. In order to study the role of PLA2 in the acrosome reaction triggered by GTP gamma S, a non-hydrolizable analogue of GTP, two well-known PLA2 inhibitory reagents were used: dexamethasone (1 mM, a synthetic glucocorticoid), and 2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid (ONO-RS-082, 320 micrograms/ml). Normal human spermatozoa were incubated for 3 h under capacitating conditions and treated with several reagents [GTP gamma S, dexamethasone, ONO-RS-082, arachidonic acid (AA) and lysophosphatidylcholine (LPC)], alone or in different combinations. In confirmation of earlier reports, GTP gamma S induced the acrosome reaction. On the other hand, dexamethasone and ONO-RS-082 were both able to inhibit the acrosome reaction induced by GTP gamma S. However, when AA or LPC was added after dexamethasone or ONO-RS-082, the acrosome reaction reached values close to those obtained using GTP gamma S alone. It is concluded that PLA2 probably plays an active role in the acrosome reaction triggered by GTP-binding proteins.

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Citations

Apr 20, 2004·Biology of the Cell·Manabu KurokawaRafael A Fissore
Oct 30, 2004·Molecular Reproduction and Development·Elisa Olivia PietrobonMiguel Walter Fornés
Dec 25, 2015·Reproduction in Domestic Animals = Zuchthygiene·M C Gramajo-BühlerG Sánchez-Toranzo
Nov 7, 2000·Biochimica Et Biophysica Acta·F M Flesch, B M Gadella

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