PMID: 3757547Aug 1, 1986Paper

Acrylamide and iodide fluorescence quenching as a structural probe of tryptophan microenvironment in bovine lens crystallins

Current Eye Research
S R PhillipsR F Borkman

Abstract

Fluorescence quenching using acrylamide and iodide quenchers has been used to investigate the microenvironments of tryptophan residues in bovine alpha-, beta-, and gamma-crystallin fractions. Acrylamide quenching is very sensitive to the degree of tryptophan accessibility to the solvent containing the acrylamide. Since acrylamide is able to diffuse into the interior of the protein, accessibility to acrylamide may result from Trp residues lying at the surface of the protein or from the existence of channels leading to the interior of the protein. Iodide ion is hydrated and is limited by its large size and charge to quenching of tryptophan residues lying at or near the surface of proteins. Tryptophan residues in the lens crystallin fractions were found to be highly accessible to acrylamide, yet the rate of quenching by acrylamide was very low, indicating that the tryptophan residues of the lens crystallin fractions occupy predominately hydrophobic environments. The high accessibility to acrylamide likely results from diffusion of acrylamide into the interior of the protein. Accessibility to iodide was much lower, as was the rate of quenching by iodide, adding support to the conclusions from acrylamide quenching.

References

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Citations

Aug 4, 2010·The Journal of Biological Chemistry·Neeraj KapoorThomas P Sakmar
Mar 1, 1988·Current Eye Research·R C AugusteynR Seifert
Jan 1, 1988·Current Eye Research·S R Phillips, R F Borkman
Mar 6, 2012·International Journal of Biological Macromolecules·Jose A MendozaGustavo Zardeneta
Aug 22, 2013·Protein Science : a Publication of the Protein Society·Zhen SongBinsheng Yang
Jun 27, 2013·Proteins·B D PierceBeverly Wendland
Jul 15, 1987·Biochemical and Biophysical Research Communications·R E HayJ M Petrash
Apr 1, 1989·Photochemistry and Photobiology·D H TallmadgeR F Borkman
Aug 5, 2015·The Protein Journal·Bahram Pooreydy GolakiMohammadsadegh Nadimifar
Apr 2, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Nicoleta StănciucGabriela Râpeanu
Jan 7, 2015·Colloids and Surfaces. B, Biointerfaces·Chih-Kai ChangSteven S-S Wang
Oct 7, 2014·Bioorganic & Medicinal Chemistry·Poulami TalukderSidney M Hecht
Aug 15, 2013·Journal of the American Chemical Society·Shengxi ChenSidney M Hecht
Apr 8, 2017·The Journal of Physical Chemistry. B·Priyanka Singh, Pramit K Chowdhury
Sep 28, 1987·FEBS Letters·R C Augusteyn, J F Koretz
Sep 20, 2018·The Journal of Biological Chemistry·Benedikt WeberJohannes Buchner
Dec 15, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Nilanjan DeySantanu Bhattacharya
Aug 25, 2019·International Journal of Biological Macromolecules·K O MuranovM A Ostrovsky
May 9, 2001·Biochimica Et Biophysica Acta·M D BazziA S Duhaiman
Mar 5, 1993·Biochimica Et Biophysica Acta·R C AugusteynT Putilina
Mar 1, 1988·Experimental Eye Research·C SlingsbyT L Blundell
May 12, 2009·Biochimica Et Biophysica Acta·Debadrita MukherjeePinak Chakrabarti

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