Acrylamide-induced molecular mutation spectra at HPRT locus in human promyelocytic leukaemia HL-60 and NB4 cell lines

Mutagenesis
Lin AoJia Cao

Abstract

Acrylamide (AA) is a compound widely used in many industries around the world. The recent finding that it is formed naturally in foods by heating raises human health concerns. AA is a proven carcinogen in animals and a probable carcinogen in humans, while its mutagenicity detected using in vitro mammalian gene mutation assays is still inconsistent in different cell systems. In the present study, we investigated the mutagenicity of AA in human promyelocytic leukaemia cells, HL-60 and NB4 cells, by examining the mutations at the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene locus. In a 6-h treatment without the exogenous activation, AA exerted a weak mutagenic effect at the highest concentration used in the study (700 mg/l) in HL-60 cells (P < 0.01) as well as in NB4 cells (P < 0.05). Molecular analysis of AA-induced mutants revealed a different mutation spectrum, when compared to that of spontaneous mutants. The most frequent spontaneous mutations were point mutations, whereas AA-induced mutations were mainly single exon deletions besides point mutations, and an increase in the proportion of partial deletion was associated with the increase of AA treatment. There was no obvious difference in the mutation spectra obs...Continue Reading

References

Apr 1, 1990·Genomics·A EdwardsW Ansorge
Jul 1, 1989·Journal of Occupational Medicine. : Official Publication of the Industrial Medical Association·J J CollinsL J Lucas
Dec 1, 1985·Mutation Research·K Hashimoto, H Tanii
Aug 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M A FriedmanM A Stedham
Mar 1, 1995·Mutation Research·M L GarriottL S Schwier
Aug 17, 1999·Occupational and Environmental Medicine·G M MarshL C Schall
Nov 24, 1999·Chemical Research in Toxicology·S C SumnerB I Ghanayem
May 29, 2000·Reproductive Toxicology·R W TylM A Friedman
Mar 27, 2002·Biological & Pharmaceutical Bulletin·Fusako NagaiHiro-Omi Tamura
Aug 9, 2002·Journal of Agricultural and Food Chemistry·Eden TarekeMargareta Törnqvist
Jan 28, 2003·Mutation Research·Birgit PaulssonMargareta Törnqvist
Oct 21, 2003·Chemical Research in Toxicology·Gonçalo Gamboa da CostaDaniel R Doerge
Jan 26, 2005·Mutation Research·Jerry M Rice
Jan 26, 2005·Mutation Research·Matthias BaumGerhard Eisenbrand
Jun 16, 2005·Environmental and Molecular Mutagenesis·Mugimane G ManjanathaDaniel R Doerge
Jan 3, 2006·Mutation Research·Naoki KoyamaMasamitsu Honma
Jan 28, 2006·Advances in Experimental Medicine and Biology·Margareta Törnqvist
Jan 20, 2007·Carcinogenesis·Ahmad Besaratinia, Gerd P Pfeifer
Aug 19, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Liping JiangLaifu Zhong
Nov 22, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Nan MeiTao Chen
Feb 22, 2008·Environmental Health Perspectives·Shengxue LiuJia Cao

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