PMID: 3543929Jan 1, 1987Paper

Actin and intermediate-sized filaments of the spines and cytoskeleton of Schistosoma mansoni

Parasitology Research
M K Abbas, G D Cain

Abstract

The organization of spines and filaments in whole worms and cytoskeletal fractions of adult Schistosoma mansoni was investigated. The ultrastructure of the spine revealed a closely packed filamentous organization of 3.5- to 5.6-nm elements and electron-lucent areas. Spines were surrounded at the base by electron-dense bodies and membrane invaginations, and covered at the tip by the syncytial surface membrane. Filaments, 7.5-11.1 nm in diameter, were closely associated with the base of the spines, between muscles, near mitochondria or nuclei, and in spaces of the subtegument. Cytoskeletal fractions prepared by homogenizing adults in Tris-HCl buffer, containing 0.6 M KCl and 1.0% Triton X-100, represented 19%-25% and 32%-38% of wet weight of males and females, respectively. The fractions contained nuclei, spines, 8 to 11 nm filaments, myofibrils, and granules. Vitellaria and egg shells were abundant in fractions from females. Six polypeptides with estimated molecular weights of 130, 96, 84, 78, 74 and 43 kdaltons were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as the major components of the cytoskeleton. Monoclonal antibody to chicken actin (MAA) was localized predominantly in surface spines and tuber...Continue Reading

References

Jan 1, 1976·Journal of Supramolecular Structure·S BrownJ A Spudich
Dec 1, 1985·Molecular and Biochemical Parasitology·A H DavisP Klich
Sep 15, 1973·Journal of Molecular Biology·F W Studier
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Aug 1, 1982·The Journal of Cell Biology·J C SamuelsonJ R David
Dec 1, 1982·The Journal of Cell Biology·C CohenM Stirewalt
Jul 1, 1980·European Journal of Immunology·D SnaryJ A Clegg

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Citations

Jan 1, 1992·Parasitology Research·A W StittS M Anderson
May 1, 1990·International Journal for Parasitology·A N MacGregor, S J Shore
Jul 1, 1995·International Journal for Parasitology·P Sobhon, W Apinhasmit
Aug 1, 1994·International Journal for Parasitology·C M DiogoM Tendler
Apr 1, 1992·Molecular and Biochemical Parasitology·R E Blanton, L S Licate
Dec 1, 1995·Molecular and Biochemical Parasitology·G C Oliveira, W M Kemp
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M K AbbasG D Cain
Nov 26, 2003·International Journal for Parasitology·Geoffrey N GobertMalcolm K Jones
Aug 6, 2013·Journal of Proteome Research·Min ZhangJiaojiao Lin
Jun 29, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Malcolm K JonesDonald P McManus
May 5, 1988·Nature·Y MatsumotoM Aikawa
Apr 24, 2001·Journal of Helminthology·S C CohenM de Fatima Diniz Baptista-Farias
Aug 21, 2013·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Stephen M HowellMaury L Hull
Nov 30, 2019·Parasitology Research·Larisa G Poddubnaya, David I Gibson

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