Actin and villin compartmentation during ATP depletion and recovery in renal cultured cells

Kidney International
N GolenhofenL J Mandel

Abstract

ATP-depletion in renal cultured cells has been used as a model for studying various cytoskeletal and functional alterations induced by renal ischemia. This communication explores the reversibility of these effects utilizing a novel method [1] that depleted ATP (ATP-D) to 2% of control within 30 minutes and caused complete recovery (REC) of ATP in one hour. Under confocal microscopy, ATP-D (30 min) caused thinning of F-actin from the microvilli, cortical region, and basal stress fibers, with the concurrent appearance of intracellular F-actin patches. These changes were more pronounced after 60 minutes of ATP-D. One hour of REC following 30 minutes of ATP-D produced complete recovery of F-actin in each region of the cell. However, after 60 minutes of ATP-D, a heterogeneous F-actin recovery pattern was observed: almost complete recovery of the apical ring and microvilli, thinned cortical actin with occasional breaks along the basolateral membrane, and a dramatic reduction in basal stress fiber density. The time course of cortical actin and actin ring disruption and recovery coincided with a drop recovery in the transepithelial resistance and the cytoskeletal dissociation and reassociation of the Na,K-ATPase. Additionally, the micr...Continue Reading

References

Oct 1, 1992·Kidney International·P S Kellerman, R T Bogusky
Oct 1, 1989·The Journal of Clinical Investigation·B A MolitorisR H Dahl
Feb 1, 1989·The Journal of Cell Biology·L M Coluccio, A Bretscher
Dec 1, 1985·The Journal of Clinical Investigation·B A MolitorisF R Simon
Sep 1, 1994·The American Journal of Physiology·J ChenL J Mandel
Oct 1, 1994·The American Journal of Physiology·C E Kobryn, L J Mandel
Apr 1, 1993·The American Journal of Physiology·R B DoctorL J Mandel

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Citations

Jun 19, 1998·Journal of Cellular Biochemistry·E A PapakonstantiC Stournaras
May 5, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R B DoctorJ G Fitz
Aug 16, 2002·Molecular Biology of the Cell·Evangelia A Papakonstanti, Christos Stournaras
Feb 13, 2001·Molecular Biology of the Cell·A JahrausG Griffiths
Sep 24, 1999·Current Opinion in Nephrology and Hypertension·S L Ashworth, B A Molitoris
Aug 18, 2004·Biochemical and Biophysical Research Communications·Sathish K GeorgeSanjay K Nigam
Jul 26, 2005·Experimental Cell Research·Artemissia-Phoebe NifliElias Castanas
Feb 26, 2003·Cell Motility and the Cytoskeleton·Luo GuJing Chen
Aug 22, 2006·The Journal of Biological Chemistry·Alok TomarSeema Khurana
Jan 12, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kimberley LewisDerek M McKay
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Jose Luis ViñasAnna Sola
Dec 3, 2003·American Journal of Physiology. Cell Physiology·Priya PrahaladKarl S Matlin
Sep 12, 2003·American Journal of Physiology. Cell Physiology·Yong QianBing-Hua Jiang
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maeilena KampaElias Castanas
May 2, 2003·American Journal of Physiology. Renal Physiology·Mauro BustamanteEric Feraille
Jun 11, 1999·The American Journal of Physiology·O AharonovitzS Grinstein
Jun 3, 2000·American Journal of Physiology. Renal Physiology·P WhiteJ Chen
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Klaus Lange
Apr 24, 2003·American Journal of Physiology. Renal Physiology·Flavia A WaldPedro J I Salas
May 12, 1998·The American Journal of Physiology·Y ZhangA A McDonough
Nov 8, 2001·American Journal of Physiology. Cell Physiology·S Herget-RosenthalB A Molitoris
Feb 12, 1998·The American Journal of Physiology·R B DoctorL J Mandel
Feb 12, 1998·The American Journal of Physiology·C AufrichtS K Van Why
Oct 25, 2002·Journal of the American Society of Nephrology : JASN·Eric A SheldenFiona M Pollock
Feb 9, 2000·Journal of the American Society of Nephrology : JASN·William E SmoyerRainer Benndorf

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