PMID: 2112547Jun 1, 1990Paper

Actin assembly in electropermeabilized neutrophils: role of intracellular calcium

The Journal of Cell Biology
G P DowneyS Grinstein

Abstract

Assembly of microfilaments involves the conversion of actin from the monomeric (G) to the filamentous (F) form. The exact sequence of events responsible for this conversion is yet to be defined and, in particular, the role of calcium remains unclear. Intact and electropermeabilized human neutrophils were used to assess more directly the role of cytosolic calcium [( Ca2+]i) in actin assembly. Staining with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and right angle light scattering were used to monitor the formation of F-actin. Though addition of Ca2+ ionophores can be known to induce actin assembly, the following observations suggest that an increased [Ca2+]i is not directly responsible for receptor-induced actin polymerization: (a) intact cells in Ca2(+)-free medium, depleted of internal Ca2+ by addition of ionophore, responded to the formyl peptide fMLP with actin assembly despite the absence of changes in [Ca2+]i, assessed with Indo-1; (b) fMLP induced a significant increase in F-actin content in permeabilized cells equilibrated with medium containing 0.1 microM free Ca2+, buffered with up to 10 mM EGTA; (c) increasing [Ca2+]i beyond the resting level by direct addition of CaCl2 to permeabilized cells resulted in actin disasse...Continue Reading

References

Oct 31, 1989·Biochemical and Biophysical Research Communications·G P DowneyS Grinstein
Feb 1, 1989·The Journal of Cell Biology·J H HartwigT P Stossel
Aug 1, 1989·European Journal of Clinical Investigation·D P Lew
Apr 14, 1989·Biochemical and Biophysical Research Communications·G P Downey, S Grinstein
May 1, 1989·British Journal of Pharmacology·P G Hellewell, T J Williams
Sep 1, 1989·The Journal of Cell Biology·S Therrien, P H Naccache
Apr 1, 1984·The Journal of Cell Biology·T H Howard, W H Meyer
Sep 1, 1984·The Journal of Cell Biology·P J WallaceM A Lichtman
Jan 29, 1980·Biochemical and Biophysical Research Communications·O I Stendahl, T P Stossel

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Citations

Jan 1, 1997·Cell Motility and the Cytoskeleton·E SuzakiS Terakawa
Feb 5, 2000·Cell Motility and the Cytoskeleton·F RichelmeP Bongrand
Dec 15, 1996·Journal of Cellular Biochemistry·M OkuyamaM Monden
Feb 1, 1996·Journal of Cellular Physiology·C J CornishC L Geczy
Jul 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M B Hallett
Jan 1, 1993·Cell Motility and the Cytoskeleton·J S WangK S Zaner
Apr 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C GarciaL F Bonewald
Jun 1, 1993·Journal of Cellular Physiology·G P DowneyC K Chan
Apr 1, 1995·Journal of Cellular Physiology·G P DowneyC K Chan
Feb 1, 1994·Current Opinion in Immunology·G P Downey
Dec 1, 1994·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M MarinovichC L Galli
Jun 14, 2005·Biochemical and Biophysical Research Communications·Neenu SinghAndrew W Thomas
Sep 1, 1991·The Journal of Cell Biology·G P DowneyG S Worthen
Jan 1, 1997·Molecular Biology of the Cell·J S WangK S Zaner
Jun 1, 2006·Acta Physiologica·C Stock, A Schwab
May 29, 2007·Infection and Immunity·Jens Martin HerrmannElizabeth R Simons
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Vladyslav DrevalAlbrecht Schwab
May 2, 2009·American Journal of Respiratory and Critical Care Medicine·Stefanos VoglisHaibo Zhang
Mar 1, 1995·Pflügers Archiv : European journal of physiology·A WiltinkB Van Duijn
Sep 13, 2007·Immunopharmacology and Immunotoxicology·M A PanaroV Mitolo
Jul 11, 2014·Expert Opinion on Drug Delivery·Maša Kandušer, Marko Ušaj
Nov 1, 2006·Critical Care Medicine·Ziqiang DingThomas K Waddell
Aug 18, 2010·Communicative & Integrative Biology·Pontus Nordenfelt, Hans Tapper
Oct 11, 1996·Biochimica Et Biophysica Acta·C Geczy
Jun 16, 1995·Cell·M P PeppelenboschJ L Bos
Oct 31, 2000·Microscopy Research and Technique·T A MillerE R Kokoska
Dec 20, 2017·Frontiers in Molecular Neuroscience·Yanfei WangZhigang Xu
Apr 20, 2010·Journal of Leukocyte Biology·Paula Nunes, Nicolas Demaurex
Apr 21, 2011·Journal of Leukocyte Biology·Pontus Nordenfelt, Hans Tapper

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