Actin-binding proteins in a postsynaptic preparation: Lasp-1 is a component of central nervous system synapses and dendritic spines

Journal of Neuroscience Research
Greg R PhillipsDavid R Colman

Abstract

CNS synapses are complex sites of cell-cell communication. Identification and characterization of the protein components of synapses will lead to a better understanding of the mechanisms of neurotransmission and plasticity. We applied multidimensional protein identification technology (MudPIT) to purified, guanidine-solubilized postsynaptic fractions to identify novel synaptically localized molecules. We identified several actin-associated proteins known to regulate actin polymerization and control cell motility in nonneural cells that have not previously been associated with CNS synaptic function. One of these is lasp-1, an actin-associated LIM and SH3 domain-containing protein. We show that lasp-1 is strongly expressed by CNS neurons and is concentrated at synaptic sites. Overall, the preponderance of actin-associated proteins in postsynaptic density fractions, and specifically those involved in actin reorganization, suggests that there are many modes by which the state of synaptic F-actin polymerization and, hence, synaptic physiology are affected.

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Citations

May 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Catherine S ChewHan Zhang
May 3, 2013·PloS One·Vincent C K CheungKi A Goosens
Jun 17, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Pierluigi Mauri, Michaela Scigelova
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Jul 28, 2005·Journal of Neuroscience Research·Greg R PhillipsDavid R Colman
Mar 23, 2007·Human Molecular Genetics·Jennifer L StoneStanley F Nelson
Oct 1, 2020·Molecular Biology of the Cell·Stephanie L PollittJames Q Zheng

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Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.