Actin-containing microprocesses in the fusion of cultured chick myoblasts

Muscle & Nerve
H L HuangE Lazarides

Abstract

Scanning electron microscopic studies of myoblasts from 11- to 13-day-old chick embroyonic breast muscle cultured on collagen-coated glass coverslips showed six stages of development into multinucleated myotubes: (1) growth of flattened, spread-out cells for 20-30 hr following initiation of monolayer cultures; (2) extension of microprocesses (1-150 microM) from cells that have become spindle shaped; (3) contact and adherence of microprocesses from adjacent cells; (4) thickening of fused processes; (5) approximation of the cells; and (6) coalescence of the cells to form a spindle-shaped myotube. When the calcium-ion concentration in the growth medium was lowered--either by increasing the concentration of ethylene-glycol-bis(aminoethyl ether)N,N'-tetraacetate (EGTA)or by decreasing the cconcentration of free calciumion used--the number of microprocesses present on the cells was reduced. Presumably, however, these microprocesses could still fuse together, provided that the calciumion concentration was greater than 160 microM. Indirect immunofluorescence assay with actin-specific antibody indicated that actin is a major component of the myoblasts' microprocesses. Cytochalasin B (5 microgram/ml) caused the microprocesses to retract ...Continue Reading

References

Oct 1, 1976·The Journal of Cell Biology·R R Weihing
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·H HoltzerA P Somlyo
Jul 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Lazarides
Dec 11, 1975·Developmental Biology·E K Bayne, S B Simpson
Feb 1, 1976·Developmental Biology·P S Moss, R C Strohman
Feb 1, 1976·Developmental Biology·B M Vertel, D A Fischman
May 1, 1976·The Journal of Cell Biology·G Albrecht-Buehler
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, K Weber
Aug 1, 1974·The Journal of Cell Biology·A F MirandaS W Tanenbaum
Oct 1, 1968·Journal of Cellular Physiology·J R Coleman, A W Coleman
May 1, 1971·Developmental Biology·A ShainbergD Yaffe
Oct 1, 1974·Experimental Cell Research·R RajaramanA Rembaum
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J W Sanger
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B M PatersonD Yaffe
May 1, 1972·The Journal of Cell Biology·B H Lipton, I R Konigsberg
Oct 1, 1972·Developmental Biology·B Paterson, R C Strohman
Nov 1, 1967·The Journal of Cell Biology·Y ShimadaA A Moscona
Jan 1, 1972·The Journal of Cell Biology·M C O'Neill, F E Stockdale
Oct 1, 1969·Experimental Cell Research·H Dalen, P Scheie
Nov 1, 1969·Experimental Cell Research·A ShainbergD Yaffe
Jan 1, 1970·Experimental Cell Research·E A Follett, R D Goldman
Sep 1, 1970·Developmental Biology·C Richler, D Yaffe
Jan 15, 1971·Science·N K WessellsK Yamada
Feb 1, 1969·Journal of Cellular Physiology·M O'Neill, R C Strohman
Mar 1, 1963·Developmental Biology·A C TAYLOR, E ROBBINS
Jul 1, 1961·The Anatomical Record·W G COOPER, I R KONIGSBERG
Jan 1, 1965·The American Journal of Anatomy·E R ALLEN, F A PEPE

❮ Previous
Next ❯

Citations

Feb 5, 2008·In Vitro Cellular & Developmental Biology. Animal·Michele L MarquetteMarguerite Sognier
Jun 1, 1980·The Journal of Cell Biology·R G MacBride, R J Przybylski
May 1, 1981·Muscle & Nerve·D Allbrook
Dec 29, 2020·Biochemical Society Transactions·Gal HaimovichJeffrey E Gerst

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.