Actin cytoskeleton controls movement of intracellular organelles in pancreatic duct epithelial cells.

Cell Calcium
Seung-Ryoung JungDuk-Su Koh

Abstract

In most eukaryotic cells, microtubules and filamentous actin (F-actin) provide tracks on which intracellular organelles move using molecular motors. Here we report that cytoplasmic movement of both mitochondria and lysosomes is slowed by F-actin meshwork formation in pancreatic duct epithelial cells (PDEC). Mitochondria and lysosomes were labeled with fluorescent Mitotracker Red CMXRos and Lysotracker Red DND-99, respectively, and their movements were monitored using epi-fluorescence and confocal microscopy. Mitochondria and lysosomes moving actively at rest stopped rapidly within several seconds after an intracellular Ca(2+) rise induced by activation of P2Y(2) purinergic receptors. The 'freezing' of the organelles was inhibited by blocking the Ca(2+) rise or by pretreatment with latrunculin B, an inhibitor of F-actin formation. Indeed, this freezing effect on the organelles was accompanied by the formation of F-actin in the whole cytoplasm as stained with Alexa 488-phalloidin in fixed PDEC. For real-time monitoring of F-actin formation in live cells, we expressed sGFP-fimbrin actin binding domain2 (fABD2) in PDEC. Rapid recruitment of the fluorescent probe near the nucleus and lysosomes suggested dense F-actin formation aroun...Continue Reading

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Citations

Feb 28, 2016·Current Opinion in Cell Biology·Saara Hämälistö, Marja Jäättelä
Feb 7, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anna Maria PosadinoGianfranco Pintus
Nov 6, 2018·Genome Génome / Conseil National De Recherches Canada·Baojin YaoYu Zhao

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