PMID: 9175265Jan 1, 1996Paper

Actin cytoskeleton demonstration in Trichomonas vaginalis and in other trichomonads

Biology of the Cell
G BrugerolleG Coffe

Abstract

The flagellate form of Trichomonas vaginalis (T v) transforms to amoeboid cells upon adherence to converslips. They grow and their nuclei divide without undergoing cytokinesis, yielding giant cells and a monolayer of T v F-actin was demonstrated in Trichomonas vaginalis by fluorescence microscopy using phalloidin and an anti-actin mAb which labelled the cytoplasm of both the flagellate and amoeboid forms. Comparative electrophoresis and immunoblotting established that the actin band has the same 42 kDa as muscle actin, but 2-D electrophoresis resolved the actin band into four spots; the two major spots observed were superimposable with major muscle actin isoforms. Electron microscopy demonstrated an ectoplasmic microfibrillar layer along the adhesion zone of amoeboid T v adhering to coverslips. Immunogold staining, using anti-actin monoclonal antibodies demonstrated that this layer was mainly composed of actin microfilaments. A comparative immunoblotting study comprising seven trichomonad species showed that all trichomonads studied expressed actin. The mAb Sigma A-4700 specific for an epitope on the actin C-terminal sequence labelled only actin of Trichomonas vaginalis, Tetratrichomonas gallinarum. Trichomitus batrachorum and ...Continue Reading

Citations

May 22, 2013·International Journal for Parasitology·Sven B GouldWilliam F Martin
Apr 23, 2003·Experimental Parasitology·Sheila B LeckeGeraldo A De Carli
Nov 6, 1998·Molecular and Biochemical Parasitology·G BricheuxG Brugerolle
Jun 12, 2008·The Journal of Antimicrobial Chemotherapy·Pratibha TiwariMan Mohan Singh
Apr 6, 2000·The Journal of Eukaryotic Microbiology·G BrugerolleG Coffe
Aug 14, 2010·Experimental Parasitology·Ana María CevallosRoberto Hernández
Dec 16, 2006·The Journal of Eukaryotic Microbiology·Christophe DuboucherEric Viscogliosi
Oct 13, 2006·Biology of the Cell·Antonio Pereira-Neves, Marlene Benchimol
Apr 4, 2015·Protist·Luiz Carlos RibeiroMarlene Benchimol
May 5, 2009·Progress in Histochemistry and Cytochemistry·Wanderley de SouzaNarcisa L Cunha-e-Silva
Apr 4, 2017·Biology of the Cell·Ivone de Andrade RosaMarlene Benchimol
Dec 12, 2019·Frontiers in Cellular and Infection Microbiology·Tansy C Hammarton
Aug 6, 2000·European Journal of Cell Biology·G BricheuxG Brugerolle

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