PMID: 7540746Mar 1, 1995Paper

Actin-dependent activation of ion conductances in bronchial epithelial cells

Pflügers Archiv : European journal of physiology
T HugK Kunzelmann

Abstract

Activation of Cl- and K+ channels is necessary to drive ion secretion in epithelia. There is substantial evidence from previous reports that vesicular transport and exocytosis are involved in the regulation of ion channels. In the present study we examined the role of cytoskeletal elements and components of intracellular vesicle transport on ion channel activation in bronchial epithelial cells. To this end, cells were incubated with a number of different compounds which interact with either microtubules or actin microfilaments, or which interfere with vesicle transport in the Golgi apparatus. The effectiveness of these agents was verified by fluorescence staining of cellular microtubules and actin. The function was examined in 36Cl- efflux studies as well as in whole-cell (WC) patch-clamp and cell-attached studies. The cells were studied under control conditions and after exposure to (in mmol/l) ATP (0.1), forskolin (0.01), histamine (0.01) and hypotonic bath solution (HBS, NaCl 72.5). In untreated control cells, ATP primarily activated a K+ conductance whilst histamine and forskolin induced mainly a Cl- conductance. HBS activated both K+ and Cl- conductances. Incubation of the cells with brefeldin A (up to 100 mumol/l) did not...Continue Reading

References

Jun 1, 1977·Die Naturwissenschaften·T Wieland
Feb 22, 1979·Nature·P B SchiffS B Horwitz
Jan 1, 1992·Annual Review of Physiology·A S Verkman
Apr 24, 1992·Science·N A BradburyK L Kirk
Nov 6, 1992·Science·C E Creutz
Apr 23, 1992·Nature·S A KuznetsovD G Weiss
Jun 1, 1991·The Journal of Clinical Investigation·M ShapiroJ L Madara
Nov 1, 1991·The American Journal of Physiology·H F CantielloD A Ausiello
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Aug 1, 1991·The Journal of Membrane Biology·E SchaererJ P Kraehenbuhl
Jan 1, 1990·Annual Review of Physiology·J D McCann, M J Welsh
Oct 11, 1988·Biochimica Et Biophysica Acta·H Gögelein
Mar 1, 1989·Renal Physiology and Biochemistry·E Schlatter
Oct 1, 1987·The Journal of Cell Biology·J A Cooper
Jul 24, 1986·Nature·K AktoriesE Habermann
Aug 1, 1967·The Journal of Cell Biology·G G Borisy, E W Taylor
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L S BarakW W Webb
Jan 1, 1994·American Journal of Respiratory Cell and Molecular Biology·A L CozensD C Gruenert
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·L S PrinceR B Marchase
Jan 1, 1994·Annual Review of Physiology·A P Morris, R A Frizzell
Aug 1, 1993·The American Journal of Physiology·H O'BrodovichJ Edelson
Oct 1, 1994·Pflügers Archiv : European journal of physiology·K KunzelmannR Greger
Oct 1, 1994·Pflügers Archiv : European journal of physiology·T KoslowskyK Kunzelmann
Mar 1, 1994·The American Journal of Physiology·C M FullerD J Benos
Apr 1, 1994·The American Journal of Physiology·K Weng, J B Wade
Jul 15, 1993·The Biochemical Journal·R D Burgoyne, A Morgan
Aug 1, 1993·Pflügers Archiv : European journal of physiology·R GregerC Normann
Nov 1, 1993·Trends in Cell Biology·L M Machesky, T D Poland

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Citations

Apr 1, 1996·Pflügers Archiv : European journal of physiology·D EckeR Greger
Aug 1, 1996·Pflügers Archiv : European journal of physiology·R Greger
Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Oct 1, 1996·General Pharmacology·B NiliusG Droogmans
Sep 29, 2009·Cell Calcium·Karl KunzelmannRainer Schreiber
Mar 5, 2011·The Journal of Medical Investigation : JMI·Hirotaka NishisakoHiroshi Miyamoto
Nov 12, 2010·Molecular Membrane Biology·Diana FariaMargarida D Amaral
May 7, 2013·International Journal of Molecular Sciences·Dmitri V Kravtsov, Nadia A Ameen
Oct 26, 1999·Circulation Research·A I BarakatP F Davies
Aug 24, 2006·American Journal of Physiology. Renal Physiology·C MazzochiP R Smith
Jan 5, 2002·Physiological Reviews·Karl Kunzelmann, Marcus Mall
Jul 1, 1996·Pflügers Archiv : European journal of physiology·M MallR Greger
Dec 1, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuemin TianKarl Kunzelmann
Feb 1, 1997·The American Journal of Physiology·T VoetsB Nilius
Sep 17, 2003·European Journal of Oral Sciences·Kazunobu OhshimaHideaki Shintani
Dec 1, 1996·The American Journal of Physiology·J MoránH Pasantes-Morales

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