Actin depolymerization affects stress-induced translational activity of potato tuber tissue

Plant Physiology
J K MorelliM E Vayda

Abstract

Changes in polymerized actin during stress conditions were correlated with potato (Solanum tuberosum L.) tuber protein synthesis. Fluorescence microscopy and immunoblot analyses indicated that filamentous actin was nearly undetectable in mature, quiescent aerobic tubers. Mechanical wounding of postharvest tubers resulted in a localized increase of polymerized actin, and microfilament bundles were visible in cells of the wounded periderm within 12 h after wounding. During this same period translational activity increased 8-fold. By contrast, low-oxygen stress caused rapid reduction of polymerized actin coincident with acute inhibition of protein synthesis. Treatment of aerobic tubers with cytochalasin D, an agent that disrupts actin filaments, reduced wound-induced protein synthesis in vivo. This effect was not observed when colchicine, an agent that depolymerizes microtubules, was used. Neither of these drugs had a significant effect in vitro on run-off translation of isolated polysomes. However, cytochalasin D did reduce translational competence in vitro of a crude cellular fraction containing both polysomes and cytoskeletal elements. These results demonstrate the dependence of wound-induced protein synthesis on the integrity ...Continue Reading

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Citations

Dec 23, 2009·Nature Reviews. Molecular Cell Biology·Seyun Kim, Pierre A Coulombe
Sep 21, 2000·The Plant Journal : for Cell and Molecular Biology·B L OrvarR S Dhindsa
Jul 26, 2008·Journal of Experimental Botany·Gilli Barel, Idit Ginzberg
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Jan 10, 2006·Proteomics·Patrick GiavaliscoJulia Kehr
Oct 30, 2007·Plant Physiology·Rajagopal BalasubramanianBrandon d Moore

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