Actin-depolymerizing effect of dimeric macrolides, bistheonellide A and swinholide A

Journal of Biochemistry
S SaitoH Karaki

Abstract

We compared the effects of dimeric marine toxins, bistheonellide A, and swinholide A, on actin polymerization. Bistheonellide A and swinholide A possess two identical side chains with similar structures to those of other marine toxins, mycalolide B, and aplyronine A. By monitoring changes in fluorescent intensity of pyrenyl-actin, bistheonellide A was found to inhibit polymerization of G-actin and to depolymerize F-actin in a concentration-dependent manner. The relationship between the concentration of bistheonellide A and its inhibitory activity on actin polymerization suggested that one molecule of bistheonellide A binds two molecules of G-actin. We demonstrated by SDS-PAGE that the complex of G-actin with bistheonellide A, swinholide A, or mycalolide B could not interact with myosin. No evidence was found that bistheonellide A severs F-actin at the concentrations examined (molar ratio to actin; 0. 025-2.5), while swinholide A showed severing activity, although it was weaker than that of mycalolide B. We also demonstrated that the depolymerizing effect of bistheonellide A or mycalolide B is irreversible. Bistheonellide A increased, while swinholide A decreased, the rate of nucleotide exchange in G-actin, suggesting that bindi...Continue Reading

Citations

Dec 14, 2002·Angewandte Chemie·Kap-Sun Yeung, Ian Paterson
Dec 26, 2002·Journal of Molecular Recognition : JMR·A V VeselovskyP Janssen
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Pearl P Y LieC Yan Cheng
Nov 13, 2007·Eukaryotic Cell·Mark D Lavine, Gustavo Arrizabalaga
May 19, 2010·Marine Drugs·Francesco Silvestre, Elisabetta Tosti
Oct 26, 2010·Current Opinion in Biotechnology·Amy E Wright
Sep 9, 2008·British Journal of Pharmacology·B EspiñaL M Botana
Mar 31, 2005·Chemistry & Biology·Vadim A KlenchinIvan Rayment
Jul 21, 2005·Annual Review of Neuroscience·Christian Dillon, Yukiko Goda
Mar 3, 2009·Plant & Cell Physiology·Yoshihisa OdaSeiichiro Hasezawa
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Begoña EspiñaLuis M Botana
Dec 17, 2014·Natural Product Reports·Masaki Kita, Hideo Kigoshi
Dec 14, 2004·Cell Motility and the Cytoskeleton·Fiona M Pollock, Jeremy D Pickett-Heaps
Aug 11, 2018·Chemical Communications : Chem Comm·Takayuki OhyoshiHideo Kigoshi
Feb 18, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephan HeggeFriedrich Frischknecht
Nov 24, 2018·Scientific Reports·Sarah NersesianAndrew W Craig
Jan 21, 2021·Cancer Metastasis Reviews·Carmen Ruggiero, Enzo Lalli
Nov 8, 2005·The Journal of Experimental Biology·I R AresL M Botana
Dec 3, 2019·Chemical Science·Xiaoyi WangJoshua B Edel
Jul 9, 2004·Biochemical and Biophysical Research Communications·Shin-ya SaitoYasushi Ohizumi
Nov 16, 2013·Journal of the American Chemical Society·Masaki KitaHideo Kigoshi
Aug 24, 2002·Journal of Natural Products·Ru Angelie EdradaPeter Proksch
Jan 31, 2006·Journal of Natural Products·Diaa T A Youssef, Susan L Mooberry

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.