Actin filament binding by a monomeric IQGAP1 fragment with a single calponin homology domain

Cell Motility and the Cytoskeleton
Scott C MateerG S Bloom

Abstract

IQGAP1 is a homodimeric protein that reversibly associates with F-actin, calmodulin, activated Cdc42 and Rac1, CLIP-170, beta-catenin, and E-cadherin. Its F-actin binding site includes a calponin homology domain (CHD) located near the N-terminal of each subunit. Prior studies have implied that medium- to high-affinity F-actin binding (5-50 microM K(d)) requires multiple CHDs located either on an individual polypeptide or on distinct subunits of a multimeric protein. For IQGAP1, a series of six tandem IQGAP coiled-coil repeats (IRs) located past the C-terminal of the CHD of each subunit support protein dimerization and, by extension, the IRs or an undefined subset of them were thought to be essential for F-actin binding mediated by its CHDs. Here we describe efforts to determine the minimal region of IQGAP1 capable of binding F-actin. Several truncation mutants of IQGAP1, which contain progressive deletions of the IRs and CHD, were assayed for F-actin binding in vitro. Fragments that contain both the CHD and at least one IR could bind F-actin and, as expected, removal of all six IRs and the CHD abolished binding. Unexpectedly, a fragment called IQGAP1(2-210), which contains the CHD, but lacks IRs, could bind actin filaments. IQG...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 30, 1995·FEBS Letters·J Castresana, M Saraste
Sep 20, 1996·The Journal of Biological Chemistry·S KurodaK Kaibuchi
Jun 30, 1997·The Journal of Cell Biology·A M BashourG S Bloom
Dec 31, 1997·The Journal of Biological Chemistry·M FukataK Kaibuchi
Aug 26, 1998·FEBS Letters·T StradalM Gimona
Oct 13, 1998·Current Biology : CB·M Gimona, S J Winder
Dec 29, 1998·The Journal of Biological Chemistry·Y D HoD B Sacks
Jan 26, 2002·The Journal of Biological Chemistry·Scott C MateerGeorge S Bloom
Mar 26, 2002·FEBS Letters·Mario GimonaSteven J Winder
Apr 12, 2002·The Journal of Biological Chemistry·Jennifer M Swart-MatarazaDavid B Sacks
Jul 12, 2002·Cell·Masaki FukataKozo Kaibuchi
Oct 12, 2002·Journal of Cell Science·Dorothy I MundyGeorge S Bloom
Oct 16, 2002·The Journal of Biological Chemistry·Gaelh Ouengue MbeleJacques Baudier
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Jun 6, 2003·Cell Motility and the Cytoskeleton·Scott C MateerGeorge S Bloom

❮ Previous
Next ❯

Citations

Sep 5, 2012·Molecular and Cellular Biochemistry·William J AndrewsDavid J Timson
Jul 7, 2011·The Journal of Biological Chemistry·Andrea Pelikan-ConchaudronMarie-France Carlier
May 25, 2010·TheScientificWorldJournal·Mahasin Osman
May 9, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·M BhattacharyaD Sheppard
Aug 5, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Galina S BogatkevichRichard M Silver
Sep 2, 2010·PloS One·Hélène FoussardFrançois Payre
Nov 8, 2011·Journal of Structural Biology·Qian ChenThomas D Pollard
Mar 10, 2009·Cellular Signalling·Michael JohnsonBeric R Henderson
Oct 28, 2008·Current Opinion in Structural Biology·Björn SjöblomKristina Djinović-Carugo
Nov 2, 2007·EMBO Reports·Dominique T Brandt, Robert Grosse
Apr 22, 2006·Journal of Molecular Biology·Vitold E GalkinEdward H Egelman
Mar 4, 2015·Molecular Immunology·Alex M AbelSubramaniam Malarkannan
Mar 25, 2011·Biochemical Society Transactions·Sevvel PathmanathanDavid J Timson
Jan 27, 2015·Trends in Cell Biology·Jessica M SmithDavid B Sacks
Dec 2, 2006·Cell Calcium·Maria I PsathaStephen R Bolsover
Nov 1, 2005·FEBS Letters·Kentaro NakanoOsamu Numata
Jun 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Huan-Yu ZhaoEn-Hua Wang
Jun 29, 2013·International Journal of Molecular Sciences·Zhiguo YuanWen Tan
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Jan 21, 2011·Molecular and Cellular Biochemistry·Seung Joon LeeSangmyung Rhee
May 11, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jacquelyn A GormanDaniel D Billadeau
Feb 22, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Subramaniam MalarkannanMonica S Thakar
Oct 18, 2019·Ocular Immunology and Inflammation·Elizabeth P ShenFung-Rong Hu
Jul 1, 2008·Molecular and Cellular Biochemistry·Sevvel PathmanathanDavid J Timson
Oct 20, 2020·Frontiers in Cell and Developmental Biology·Thibaud RivièreDaniela Maier-Begandt
Feb 27, 2020·Cell Reports·Xiaohua DongZhiyong Shao
Feb 1, 2007·Journal of Cell Science·Lorena B BenseñorGeorge S Bloom
Jul 14, 2011·Journal of Cell Science·Ana Ruiz-SáenzIsabel Correas
Jan 15, 2014·Journal of Cell Science·Stephen G Naylor, David O Morgan
Dec 10, 2016·Journal of Proteome Research·Niramol JitprasutwitJoanne M Stevens
Nov 28, 2021·International Journal of Molecular Sciences·Niloufar MosaddeghzadehMohammad R Ahmadian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.