PMID: 9658181Jul 11, 1998Paper

Actin filaments and microtubules are involved in different membrane traffic pathways that transport sphingolipids to the apical surface of polarized HepG2 cells

Molecular Biology of the Cell
M M ZegersD Hoekstra

Abstract

In polarized HepG2 hepatoma cells, sphingolipids are transported to the apical, bile canalicular membrane by two different transport routes, as revealed with fluorescently tagged sphingolipid analogs. One route involves direct, transcytosis-independent transport of Golgi-derived glucosylceramide and sphingomyelin, whereas the other involves basolateral to apical transcytosis of both sphingolipids. We show that these distinct routes display a different sensitivity toward nocodazole and cytochalasin D, implying a specific transport dependence on either microtubules or actin filaments, respectively. Thus, nocodazole strongly inhibited the direct route, whereas sphingolipid transport by transcytosis was hardly affected. Moreover, nocodazole blocked "hyperpolarization," i.e., the enlargement of the apical membrane surface, which is induced by treating cells with dibutyryl-cAMP. By contrast, the transcytotic route but not the direct route was inhibited by cytochalasin D. The actin-dependent step during transcytotic lipid transport probably occurs at an early endocytic event at the basolateral plasma membrane, because total lipid uptake and fluid phase endocytosis of horseradish peroxidase from this membrane were inhibited by cytochal...Continue Reading

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Citations

Jan 17, 2002·Current Opinion in Cell Biology·Dorothy A Schafer
Feb 17, 2000·Trends in Cell Biology·S Ellis, H Mellor
Oct 8, 1999·Molecular Biology of the Cell·S C van IJzendoorn, D Hoekstra
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Apr 1, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Pavla PlačkováHelena Mertlíková-Kaiserová
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Oct 1, 2008·Biochemical and Biophysical Research Communications·Xiaoling GaoXinguo Jiang
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Aug 21, 2021·Nature Chemical Biology·David F CaianielloDavid A Spiegel

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