Actin filaments modulate hypoosmotic-responsive K+ efflux channels in specialised cells of developing bean seed coats

Functional Plant Biology : FPB
Wen-Hao ZhangStephen D Tyerman

Abstract

In developing bean (Phaseolus vulgaris L.) seeds, nutrients move in the symplasm from sieve elements to ground-parenchyma cells where they are transported across the plasma membrane into the seed apoplasm. Release of nutrients to the seed apoplasm is related to the osmotic conditions of the apoplasm. A hypoosmotic solution, resulting from enhanced uptake of nutrients by cotyledons, stimulates nutrient release from seed coat to the apoplasm. We investigated hypoosmotic nutrient release by examining the ionic membrane currents that respond to hypoosmotic treatment in protoplasts derived from three important cell types that occur at the seed coat-cotyledonary boundary. A non-selective but predominantly K+ efflux current that displayed a distinct time-dependent inactivation was elicited by membrane depolarisation under hypoosmotic conditions only in ground-parenchyma protoplasts. Hypoosmotic treatment had little effect on whole-cell ionic currents in protoplasts derived from coat chlorenchyma cells and cotyledon dermal cells. The inactivating K+ efflux current was elicited under isosmotic conditions by treatment with cytochalasin D, which disrupts actin filaments. Hypoosmotic treatment and cytochalasin D failed to induce the K+ cur...Continue Reading

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Citations

Nov 10, 2009·Plant, Cell & Environment·Natasha L Teakle, Stephen D Tyerman

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