Actin Filaments Regulate Exocytosis at the Hair Cell Ribbon Synapse

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Marie GuilletRégis Nouvian

Abstract

Exocytosis at the inner hair cell ribbon synapse is achieved through the functional coupling between calcium channels and glutamate-filled synaptic vesicles. Using membrane capacitance measurements, we investigated whether the actin network regulates the exocytosis of synaptic vesicles at the mouse auditory hair cell. Our results suggest that actin network disruption increases exocytosis and that actin filaments may spatially organize a subfraction of synaptic vesicles with respect to the calcium channels. Significance statement: Inner hair cells (IHCs), the auditory sensory cells of the cochlea, release glutamate onto the afferent auditory nerve fibers to encode sound stimulation. To achieve this task, the IHC relies on the recruitment of glutamate-filled vesicles that can be located in close vicinity to the calcium channels or more remotely from them. The molecular determinants responsible for organizing these vesicle pools are not fully identified. Using pharmacological tools in combination with membrane capacitance measurements, we show that actin filament disruption increases exocytosis in IHCs and that actin filaments most likely position a fraction of vesicles away from the calcium channels.

Citations

Aug 20, 2016·ELife·Jason Seth RothmanRobin Angus Silver
Aug 2, 2018·Physiological Reviews·Tina PangrsicAlexandra Koschak
May 6, 2019·International Journal of Molecular Sciences·Rituparna Chakrabarti, Carolin Wichmann
Jun 11, 2019·ELife·Savani AnbalaganGil Levkowitz
Aug 3, 2019·Physiological Reviews·Tobias MoserFrank Schmitz
Nov 25, 2020·International Journal of Molecular Sciences·Roos Anouk Voorn, Christian Vogl

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