PMID: 42005Jan 1, 1978

Actin from diploid and haploid cells of the myxomycete Physarum flavicomum--comparison with skeletal muscle actin

H R Henney, C Hubbell


Glycerinated diploid microplasmodia of Physarum flavicomum exhibited ATP-dependent contractility typical of actomyosin controlled systems. Actin was isolated from microplasmodia, haploid amoebae-swarm cells, and rabbit skeletal muscle, by classical methods as well as ATP-DEAE cellulose chromatography, and isoelectric focusing. Actins from all three sources had isoelectric points in the pH 6.5-7.0 range, and behaved similarly during the various isolation procedures. SDS-gel electrophoresis revealed that the actin from microplasmodia and muscle had a molecule weight of about 46,000 daltons while the amoebae-swarm cells possessed proteins of molecular weights of about 46,000 and 51,000 daltons.

Related Concepts

Chromatography, DEAE-Cellulose
Diploid Cell
Yeast Proteins
Haploid Cell
Hydrogen-Ion Concentration
Isoelectric Focusing

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