PMID: 6406516Jun 1, 1983Paper

Actin-independent association of vinculin with the cytoplasmic aspect of the plasma membrane in cell-contact areas

The Journal of Cell Biology
Z AvnurB Geiger

Abstract

We investigated the mode of association of vinculin with areas of contact between the termini of microfilament bundles and the cell membrane in sites of focal contact with the substrate by selective removal of actin from these areas. Opened-up substrate-attached membranes of chick fibroblasts as well as detergent-permeabilized cells were treated with fragmin from Physarum in the presence of Ca+2. This treatment removed actin filaments from the cytoplasmic faces of the membranes, along with several actin-associated proteins (alpha-actinin, tropomyosin, myosin, and filamin). Vinculin distribution was not affected by treatment. Moreover, rhodamine- or fluorescein-conjugated vinculin, when added to these preparations, became specifically associated with the focal contacts regardless of whether the latter were pretreated with fragmin or not. We conclude that the association of vinculin with focal contacts is largely actin-independent. We discuss the implications of these findings in the molecular mechanisms of microfilament membrane association in areas of cell contact.

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Citations

Jan 1, 1991·Cell Motility and the Cytoskeleton·B Geiger, D Ginsberg
Jan 1, 1994·Cell Motility and the Cytoskeleton·D M Morton, R Tchao
Dec 1, 1989·The Journal of Membrane Biology·B Boyer, J P Thiery
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Jan 1, 1989·Electron Microscopy Reviews·M V Nermut
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·B GeigerT Volberg
Apr 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·J L Duband, J P Thiery
Jan 5, 2002·Biomaterials·Sharon L SanbornKandice Kottke-Marchant
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Irina KaverinaJ Victor Small
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Hagmann
Oct 1, 1985·The Journal of Cell Biology·B GeigerT Volberg
Dec 1, 1989·The Journal of Cell Biology·P JonesD R Critchley
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