Actin-interacting protein 1 controls assembly and permeability of intestinal epithelial apical junctions

American Journal of Physiology. Gastrointestinal and Liver Physiology
Susana LechugaAndrei I Ivanov

Abstract

Adherens junctions (AJs) and tight junctions (TJs) are crucial regulators of the integrity and restitution of the intestinal epithelial barrier. The structure and function of epithelial junctions depend on their association with the cortical actin cytoskeleton that, in polarized epithelial cells, is represented by a prominent perijunctional actomyosin belt. The assembly and stability of the perijunctional cytoskeleton is controlled by constant turnover (disassembly and reassembly) of actin filaments. Actin-interacting protein (Aip) 1 is an emerging regulator of the actin cytoskeleton, playing a critical role in filament disassembly. In this study, we examined the roles of Aip1 in regulating the structure and remodeling of AJs and TJs in human intestinal epithelium. Aip1 was enriched at apical junctions in polarized human intestinal epithelial cells and normal mouse colonic mucosa. Knockdown of Aip1 by RNA interference increased the paracellular permeability of epithelial cell monolayers, decreased recruitment of AJ/TJ proteins to steady-state intercellular contacts, and attenuated junctional reassembly in a calcium-switch model. The observed defects of AJ/TJ structure and functions were accompanied by abnormal organization and ...Continue Reading

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Citations

Sep 15, 2016·PloS One·Swapan K DasguptaPerumal Thiagarajan
Jun 11, 2017·Cold Spring Harbor Perspectives in Biology·Miguel A GarciaNatalie Chavez
Jul 30, 2020·The Journal of Biological Chemistry·Vivian W TangWilliam M Brieher
Jun 20, 2019·Veterinary Research·Fien De MeyerFilip Van Immerseel

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