PMID: 264664Jan 1, 1977

Actin microheterogeneity in chick embryo fibroblasts

Proceedings of the National Academy of Sciences of the United States of America
P A Rubenstein, J A Spudich

Abstract

Secondary chick embryo fibroblasts contain three distinct actin species--alpha, beta, and lambda, in the approximate ratio of 1:6:3--with the same molecular weights but different isoelectric points. The most acidic of these components, alpha, comigrates on isoelectric focusing gels with the major actin of cardiac and skeletal muscle, while lambda, the most basic of the actins, comigrates with smooth muscle actin. The three components have overlapping methionine-containing tryptic peptides. All three actins are found to be present in actomyosin and cytoskeleton preparations from chick embryo fibroblasts. Identification of alpha-actin as the major actin from sarcomere-containing cells is confirmed by comparing embryonic chicken pre- and post-fusion myoblast cultures. Following myoblast fusion, the relative amount of alpha-actin increases until it changes from a minor actin component to the predominant actin species in the culture.

References

Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G PosteG L Nicolson
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R G WhalenF Gros
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R V Storti, A Rich
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J W Sanger
Jan 1, 1974·CRC Critical Reviews in Biochemistry·T D Pollard, R R Weihing

Citations

Mar 4, 1998·Cell Motility and the Cytoskeleton·D A SchaferJ A Cooper
Jul 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P A Rubenstein
Jun 1, 1984·In Vitro·S A MooreM N Hart
Feb 1, 1979·Developmental Biology·E A Fyrberg, J J Donady
Aug 1, 1981·Developmental Biology·S J TapscottH Holtzer
Feb 1, 1981·Developmental Biology·M N Woodroofe, L F Lemanski
Oct 1, 1987·Developmental Biology·C R TomlinsonW R Jeffery
Jan 1, 1980·The International Journal of Biochemistry·M L Nagpal, J C Brown
Jan 1, 1979·Neuroscience·R W LeeJ M Trifaró
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y KaufmannS Penman
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K Wang, S J Singer
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E D Korn
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Vandekerckhove, K Weber
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·V G RiddleA B Pardee
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D S DuricaW R Crain
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A Steinberg
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J L MorganB S Spooner
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B P Kaine, B B Spear
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G GabbianiW W Franke
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H M Blau, C Webster
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J C BulinskiS D Hauschka
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E Baldwin, C Kayalar
Mar 1, 1985·The Journal of Cell Biology·A M GownP L Lu
Jul 1, 1985·The Journal of Cell Biology·I M Herman, P A D'Amore
Jul 1, 1979·The Journal of Cell Biology·A Spudich, J A Spudich
Jul 1, 1979·The Journal of Cell Biology·S J HorovitchM L Pardue
Mar 1, 1980·The Journal of Cell Biology·R Bravo, J E Celis
Jan 1, 1982·The Journal of Cell Biology·P A RubensteinA Sandra
Nov 11, 1980·Nucleic Acids Research·C P OrdahlM T Largen
Feb 11, 1981·Nucleic Acids Research·M ShaniD Yaffe
Feb 15, 1992·European Journal of Biochemistry·M TerashimaM Shimoyama
Jul 1, 1978·Journal of Neurochemistry·Q L Choo, D Bray
Oct 8, 2013·PLoS Genetics·Meghan C Drummond, Karen H Friderici
Jun 1, 1978·Journal of Neurochemistry·C A MarottaJ M Gilbert
Oct 20, 1982·Biochimica Et Biophysica Acta·L A WilliamsJ Horwitz
Aug 23, 1977·Biochimica Et Biophysica Acta·F H SchachatH F Epstein
Dec 13, 1985·Biochimica Et Biophysica Acta·M Kuroda
Oct 25, 1979·Biochimica Et Biophysica Acta·J I Garrels, T Hunter
Dec 28, 1981·Biochimica Et Biophysica Acta·P A OverbeekL J Kleinsmith
Oct 1, 1978·Meat Science·N L King
Jun 9, 2007·The FEBS Journal·Wasana A K A MudaligeDavid H Heeley
Apr 1, 1979·Analytical Biochemistry·J L LessardR Akeson
Feb 28, 2014·Cytoskeleton·P A Rubenstein, Kuo-Kuang Wen
Jun 1, 1980·Experimental Cell Research·R Bravo, J E Celis
Jun 1, 1979·Experimental Cell Research·M MossR Schwartz
Feb 15, 1990·Archives of Biochemistry and Biophysics·M A VillanuevaJ L Wang
May 1, 1979·Analytical Biochemistry·J Tannenbaum, A Rich
Feb 1, 1982·Metabolism: Clinical and Experimental·R KobayashiJ B Field
Jul 1, 1979·Experimental Eye Research·G Y Mousa, J R Trevithick
Jun 1, 1980·Journal of Neurochemistry·D ReinJ L Lessard
Dec 1, 1988·Differentiation; Research in Biological Diversity·J Woodcock-MitchellG Gabbiani
Jan 1, 1981·Differentiation; Research in Biological Diversity·R ColomboG Valle
Apr 15, 1979·Archives of Biochemistry and Biophysics·P A Rubenstein, R D Ivarie
Sep 1, 1981·Archives of Biochemistry and Biophysics·P A Rubenstein
Aug 1, 1980·Archives of Biochemistry and Biophysics·J P Weir, D W Frederiksen
Nov 30, 2011·Developmental Biology·Nate WeymouthDon C Rockey
Mar 28, 1980·Biochimica Et Biophysica Acta·S SakiyamaS Fujimura
Dec 1, 1977·Cell·T G Clark, R W Merriam
Jan 1, 1989·Cell Motility and the Cytoskeleton·A K Shires, P A Rubenstein
Jan 1, 1988·Cell Motility and the Cytoskeleton·J L Lessard
Apr 1, 1979·European Journal of Biochemistry·M A KibbelaarH Bloemendal
Apr 1, 1980·European Journal of Biochemistry·I BlikstadS Eriksson
Jul 28, 2007·Tsitologiia·S Iu Khaĭtlina
Oct 17, 2019·International Journal of Molecular Sciences·Natalya Bildyug
Nov 1, 1981·Muscle & Nerve·T ObinataS Ebashi
Jun 1, 1986·Journal of Neurochemistry·A CôtéJ M Trifaró

Related Concepts

Isoactin
Actomyosin
Chick Embryo
Microtrabecular Lattice
Fibroblasts
Hybrid Cells
Isoelectric Focusing
Smooth Muscle
Muscle
Myocardium

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
R G WhalenF Gros
Proceedings of the National Academy of Sciences of the United States of America
R V Storti, A Rich
© 2021 Meta ULC. All rights reserved