Actin polymerization and ATP hydrolysis

Science
E D KornD Pantaloni

Abstract

F-actin is the major component of muscle thin filaments and, more generally, of the microfilaments of the dynamic, multifunctional cytoskeletal systems of nonmuscle eukaryotic cells. Polymeric F-actin is formed by reversible noncovalent self-association of monomeric G-actin. To understand the dynamics of microfilament systems in cells, the dynamics of polymerization of pure actin must be understood. The following model has emerged from recent work. During the polymerization process, adenosine 5'-triphosphate (ATP) that is bound to G-actin is hydrolyzed to adenosine 5'-diphosphate (ADP) that is bound to F-actin. The hydrolysis reaction occurs on the F-actin subsequent to the polymerization reaction in two steps: cleavage of ATP followed by the slower release of inorganic phosphate (Pi). As a result, at high rates of filament growth a transient cap of ATP-actin subunits exists at the ends of elongating filaments, and at steady state a stabilizing cap of ADP.Pi-actin subunits exists at the barbed ends of filaments. Cleavage of ATP results in a highly stable filament with bound ADP.Pi, and release of Pi destabilizes the filament. Thus these two steps of the hydrolytic reaction provide potential mechanisms for regulating the monomer...Continue Reading

Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·P A Dufort, C J Lumsden
Jun 11, 1999·Journal of Cellular Physiology·Klaus Lange
Aug 15, 2000·Journal of Cellular Physiology·Klaus Lange
Jan 1, 1990·Cell Motility and the Cytoskeleton·D H WangT H Howard
Jan 1, 1993·Cell Motility and the Cytoskeleton·P A Dufort, C J Lumsden
Aug 1, 1992·Protein Science : a Publication of the Protein Society·D D Root, Emil Reisler
Jan 1, 1990·Cytotechnology·R ColomboG Vailati
Aug 1, 1994·Cell Biology and Toxicology·G CappellettiR Maci
Feb 1, 1996·Pflügers Archiv : European journal of physiology·T FurukawaM Hiraoka
Jan 5, 2011·European Biophysics Journal : EBJ·Andrea VigGábor Hild
Apr 25, 2012·Protoplasma·Julie GranthamRoger Karlsson
Apr 1, 1996·International Journal of Biological Macromolecules·H H ZengY Zhang
Nov 1, 1991·Pharmacology & Therapeutics·J J Correia
Nov 1, 1989·Trends in Neurosciences·P Forscher
Sep 21, 1994·Biochimica Et Biophysica Acta·T Ohm, A Wegner
Feb 8, 1996·Biochimica Et Biophysica Acta·N SuzukiK Mihashi
Jan 1, 1996·Toxicology Letters·M G RomaE A Rodríguez Garay
Nov 19, 2003·Journal of Molecular Biology·John K Chik, David C Schriemer
Jan 15, 2004·Experimental Cell Research·Petra NiessenGiulio Gabbiani
Jan 26, 2013·Journal of the American Chemical Society·María José MayoralGustavo Fernández
Sep 22, 1999·European Journal of Biochemistry·H SchülerR Karlsson
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hyeran KangEnrique M De La Cruz
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M FlahertyK C Holmes
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C E Schutt, U Lindberg
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M F CarlierD Pantaloni
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A MukherjeeJ Lutkenhaus
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·T FinkelP J Goldschmidt-Clermont
Jun 10, 2010·The Journal of Biological Chemistry·Dmitri S KudryashovEmil Reisler
Oct 24, 2007·The Journal of Biological Chemistry·Dmitri S KudryashovKarla J F Satchell
Jun 3, 2008·The Journal of Biological Chemistry·Mitsusada IwasaToshiro Oda
Oct 1, 1993·The Journal of Cell Biology·M Fechheimer, S H Zigmond
Apr 29, 1998·Neuroreport·G MaguireH F Cantiello
Jan 1, 1991·Journal of Microscopy·J LepaultJ L Ranck
Feb 1, 1989·European Journal of Biochemistry·M Chabre, P Deterre
Jun 15, 1989·European Journal of Biochemistry·H Strzelecka-GoøaszewskaJ Moraczewska
Jul 15, 1991·European Journal of Biochemistry·Y DoiA Vertut-Doï
Feb 15, 1992·European Journal of Biochemistry·M TerashimaM Shimoyama
Nov 15, 1995·European Journal of Biochemistry·L E LahamP A Janmey
Apr 1, 1994·Brain Pathology·J A MercerS T Brady
Dec 1, 1993·Photochemistry and Photobiology·R D Ludescher, Z Liu
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Rut Carballido-López
Mar 3, 2010·Annual Review of Biophysics·Beáta Bugyi, M F Carlier
Oct 19, 2012·PloS One·Markus DüttmannAlexander S Mikhailov
Mar 27, 2012·The Journal of Chemical Physics·Mathieu CabyJean-Paul Ryckaert
Mar 25, 2011·The Journal of Chemical Physics·A N Semenov, I A Nyrkova
Oct 26, 2010·The Journal of Chemical Physics·Kunkun GuoReinhard Lipowsky
Jul 10, 2009·The Journal of Chemical Physics·Kunkun GuoReinhard Lipowsky
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dimitrios VavylonisBen O'Shaughnessy
Mar 1, 2014·Journal of Materials Science. Materials in Medicine·Stefan HaubenwallnerUte Schäfer
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J DayelR D Mullins
Oct 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·María R Gómez-García, Arthur Kornberg
Oct 3, 2014·The Journal of Chemical Physics·Yong-Woon Jung, Michael Mascagni
Sep 15, 2014·Nature Biotechnology·Yuehan FengPaola Picotti
Sep 1, 2012·Biochemistry·Ishutesh JainRanjith Padinhateeri
Feb 1, 1992·Current Opinion in Cell Biology·A Bremer, U Aebi
Jul 13, 2013·The Journal of Physical Chemistry. B·Xin Li, Anatoly B Kolomeisky
Aug 12, 2006·Physical Chemistry Chemical Physics : PCCP·Alison RodgerTimothy R Dafforn
Feb 5, 2003·The Journal of Cell Biology·Raymond S MaulDavid D Chang
Jan 1, 1995·Connective Tissue Research·P P WebbM Benjamin
Nov 19, 2015·Plant & Cell Physiology·Saku T KijimaTaro Q P Uyeda
Jan 1, 1990·Annals of the New York Academy of Sciences·Thomas D PollardP Maupin
Oct 1, 1995·Kidney International·H F Cantiello
Oct 1, 1992·Kidney International·P S Kellerman, R T Bogusky
Jan 4, 2012·Journal of Theoretical Biology·Takashi SaitouTakashi Suzuki
Nov 6, 2003·Organic & Biomolecular Chemistry·Dahui Zhao, Jeffrey S Moore
Sep 24, 2015·Journal of Proteome Research·Anke SchröderAndreas Pich
Jan 22, 2005·Molecular Microbiology·Beatriz LaraOrietta Massidda
Dec 22, 2005·The European Journal of Neuroscience·Yannan OuyangDennis W Choi
May 23, 2013·Journal of Neurochemistry·Tomoaki Shirao, Christian González-Billault
Sep 14, 2010·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Mike Bindschadler
Mar 8, 2011·The EMBO Journal·Akihiro NaritaYuichiro Maéda
Feb 28, 2014·Cytoskeleton·P A Rubenstein, Kuo-Kuang Wen
Aug 31, 2002·Biophysical Journal·Alex Mogilner, Leah Edelstein-Keshet
Apr 28, 2001·Biophysical Journal·B K BerdievI I Ismailov
Feb 24, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christopher RosinRoland Winter
Feb 12, 2008·Biochemical and Biophysical Research Communications·József OrbánGábor Hild
Feb 11, 2015·Physics of Life Reviews·Warren Colomb, Susanta K Sarkar
Mar 13, 1999·Current Biology : CB·J B Bingham, T A Schroer
Jan 18, 2003·Developmental Cell·Rut Carballido-López, Jeff Errington
Dec 30, 2008·Journal of Molecular Biology·Patrick FreireCecília Maria Arraiano
Jan 31, 2006·Biophysical Journal·Evgeny B Stukalin, Anatoly B Kolomeisky
Dec 24, 2013·Biophysical Journal·Hyeran KangEnrique M De La Cruz
May 20, 2014·European Journal of Cell Biology·Gábor HildBeáta Bugyi
Aug 17, 2011·Biophysical Journal·Kurt M SchmollerAndreas R Bausch
Jun 4, 1999·Biophysical Journal·N Fuller, R P Rand
Feb 27, 2001·Biophysical Journal·D GünzelW R Schlue
Aug 1, 1997·Journal of Structural Biology·M O SteinmetzU Aebi
Nov 23, 2016·Chemistry : a European Journal·Ludger TebbenArmido Studer
Feb 16, 2017·Journal of Neurochemistry·Tomoaki ShiraoYuko Sekino
Oct 20, 1989·Journal of Molecular Biology·C E SchuttN Strauss
Jul 24, 2010·Toxins·Karla J F Satchell
Sep 15, 2017·Molecular Biology of the Cell·Patrick R StoddardBuzz Baum
Jul 26, 1996·The Journal of Biological Chemistry·B K BerdievI I Ismailov
Aug 29, 2002·Nature Cell Biology·Ikuko FujiwaraShin'ichi Ishiwata
Aug 1, 1996·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·M TempelE Sackmann
Nov 2, 2018·Biophysics Reviews·Fikret AydinGregory A Voth
Apr 29, 1992·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M F Carlier
Aug 1, 1993·Molecular Microbiology·J Lutkenhaus
Jan 18, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Marie E BecknerIan F Pollack
May 13, 2006·The FEBS Journal·Andras MuhlradEmil Reisler
Aug 7, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Patrick W OakesJay X Tang
Feb 21, 2019·Annual Review of Biophysics·Tadej KotnikDamijan Miklavčič
Nov 6, 2009·The Journal of Biological Chemistry·Renjian HuangChih-Lueh Albert Wang
Jan 7, 2020·Royal Society Open Science·Andrew AdamatzkyFlorian Huber
Sep 22, 1995·The Journal of Biological Chemistry·C KingL Greene
Feb 1, 2003·The Journal of Biological Chemistry·Rongcun YangRichard B S Roden
May 11, 2020·Cell and Tissue Research·Awang Hazmi Awang-JunaidiAli Honaramooz
Aug 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hui JiangJianghuai Liu
Apr 1, 1992·Journal of Muscle Research and Cell Motility·E S HennesseyJ C Sparrow
Mar 29, 2003·Physiological Reviews·C G dos RemediosN J Nosworthy
Mar 19, 2016·Cold Spring Harbor Perspectives in Biology·Thomas D Pollard
Jul 19, 2018·Histochemistry and Cell Biology·Csaba BajuszPéter Vilmos
Nov 22, 2018·Biophysics Reviews·Ikuko FujiwaraYuichiro Maéda
Sep 27, 2018·Physical Review. E·Robert Marsland, Jeremy L England
Jun 1, 1992·Journal of Muscle Research and Cell Motility·J E EstesL C Gershman
Oct 17, 2001·The Journal of Biological Chemistry·M BárányK Bárány
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Klaus Lange
Jun 28, 2013·The Journal of Biological Chemistry·Marianela G DalghiJuan Pablo F C Rossi
Dec 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Thomas Niedermayer, Reinhard Lipowsky
Sep 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Clement P M ScipionRobert C Robinson
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Roberto Dominguez
Dec 29, 2019·International Journal of Molecular Sciences·L Shannon HollidayWellington J Rody
May 12, 2018·Nature Communications·Kotaro TanakaAkihiro Narita
Apr 1, 2019·Frontiers of Physics·Devin L HarrisonJun Huang
Nov 1, 1991·The American Journal of Physiology·H F CantielloD A Ausiello
Sep 4, 2020·Advanced Materials·Jie Deng, Andreas Walther
Jan 21, 2021·Cancer Metastasis Reviews·Carmen Ruggiero, Enzo Lalli
Sep 26, 2018·Biophysical Journal·Harshwardhan H KatkarGregory A Voth
Mar 4, 2021·International Journal of Molecular Sciences·Joanna Gruszczynska-BiegalaHanna Strzelecka-Gołaszewska
Apr 12, 2003·Physical Review Letters·Ben O'Shaughnessy, Dimitrios Vavylonis
Nov 18, 1996·Physical Review Letters·F AmblardS Leibler
Feb 6, 2017·Molecular and Cellular Neurosciences·Noriko KoganezawaTomoaki Shirao

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