Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin-2 and EFF-1 to Promote Membrane Repair in C. elegans.

Developmental Cell
Xinan MengSuhong Xu

Abstract

Membrane repair is essential for cell and organism survival. Exocytosis and endocytosis facilitate membrane repair in small wounds within a single cell; however, it remains unclear how large wounds in the plasma membrane are repaired in metazoans. Here, we show that wounding triggers rapid transcriptional upregulation and dynamic recruitment of the fusogen EFF-1 to the wound site in C. elegans epidermal cells. EFF-1 recruitment at the wounded membrane depends on the actin cytoskeleton and is important for membrane repair. We identified syntaxin-2 (SYX-2) as an essential regulator of EFF-1 recruitment. SYX-2 interacts with the C terminus of EFF-1 to promote its recruitment, facilitating both endoplasmic and exoplasmic membrane repair. Furthermore, we show that SYX-2-EFF-1 repair machinery acts downstream of the ESCRT III signal. Together, our findings identify a key pathway underlying membrane repair and provide insights into tissue repair and regenerative medicine after injury.

References

Mar 7, 2002·Developmental Cell·William A MohlerBenjamin Podbilewicz
Oct 29, 2002·Nature Cell Biology·William WoodPaul Martin
Jan 17, 2003·Molecular Biology of the Cell·Tatsuru TogoRichard A Steinhardt
May 9, 2003·Nature·Dimple BansalKevin P Campbell
Aug 20, 2003·The Journal of Cell Biology·Sabyasachi ChakrabartiNorma W Andrews
Mar 3, 2004·The Journal of Biological Chemistry·Swathi K RaoNorma W Andrews
Sep 3, 2004·Current Biology : CB·Gidi ShemerBenjamin Podbilewicz
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Paul L McNeil, Tom Kirchhausen
Jan 19, 2007·Molecular Biology of the Cell·Tamar GattegnoBenjamin Podbilewicz
May 4, 2007·Annual Review of Cell and Developmental Biology·Matthias Schäfer, Sabine Werner
May 10, 2007·Developmental Cell·Amir SapirBenjamin Podbilewicz
Mar 5, 2008·The Journal of Cell Biology·Vincent IdoneNorma W Andrews
Apr 9, 2008·Current Biology : CB·Nathalie PujolJonathan J Ewbank
Apr 16, 2008·Developmental Cell·Jeffrey P RasmussenJames R Priess
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Jul 28, 2009·Current Biology : CB·Andrew G ClarkWilliam M Bement
Mar 6, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anindya Ghosh-RoyAndrew D Chisholm
May 8, 2010·Science·Meital Oren-SuissaBenjamin Podbilewicz
Jun 10, 2010·The Journal of Cell Biology·Christina TamNorma W Andrews
Dec 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Taulant BacajThomas C Südhof
Jul 5, 2011·Annual Review of Cell and Developmental Biology·Kevin J Sonnemann, William M Bement
Nov 22, 2011·Current Biology : CB·Suhong Xu, Andrew D Chisholm
Feb 1, 2012·Nature Methods·Christian Frøkjær-JensenErik M Jorgensen
Mar 17, 2012·Developmental Cell·Urmas Roostalu, Uwe Strähle
Mar 30, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Andrew D Chisholm, Tiffany I Hsiao
Mar 30, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Andrew D Chisholm, Suhong Xu
Aug 30, 2013·Molecular Biology of the Cell·Rodrigo Fernandez-Gonzalez, Jennifer A Zallen
Oct 5, 2013·Scientific Reports·Stéphanie CabantousGeoffrey S Waldo
Feb 1, 2014·Science·Ana Joaquina JimenezFranck Perez
Apr 15, 2014·Cell·Jimena Pérez-VargasFélix A Rey
Aug 26, 2014·Trends in Cell Biology·Norma W AndrewsMatthias Corrotte
Oct 15, 2014·Developmental Cell·Suhong Xu, Andrew D Chisholm
Dec 24, 2014·Nature Communications·Luana L SchefferJyoti K Jaiswal
Jun 6, 2015·Genetics·Daniel J DickinsonBob Goldstein
Dec 15, 2015·Seminars in Cell & Developmental Biology·N W Andrews, F Perez
Feb 9, 2016·Cell Reports·Ksenia Smurova, Benjamin Podbilewicz
Mar 1, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Suhong XuAndrew D Chisholm

❮ Previous
Next ❯

Citations

Dec 31, 2020·STAR Protocols·Chandra Sugiarto WijayaSuhong Xu
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Weida RenJunqi Huang
Apr 9, 2021·Journal of Proteomics·Murugesan PooranachithraKrishnaswamy Balamurugan
Apr 15, 2021·BMC Biology·Dustin A AmmendoliaJohn H Brumell
Jun 3, 2021·Cells·Md Istiaq Obaidi TanvirShigehiko Yumura
Feb 4, 2021·Cell Regeneration·Yicong MaSuhong Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.