'Actin-reactive' discriminated from 'non-actin-reactive' smooth muscle autoantibody by immunofluorescence reactivity with rat epithelial cell line

Pathology
Ban-Hock TohRufus W Burlingame

Abstract

To compare smooth muscle antibody (SMA) patterns in tissue sections with patterns in an immunofluorescence assay (IFA) using a rat intestinal epithelial cell line and results from an F-actin IgG ELISA. SMA positive sera (n = 188) were classified by immunofluorescence staining of rodent kidney, stomach and liver sections as SMA-T (tubules) (n = 124) or SMA-V (vessels) (n = 64). The F-actin pattern on the rat epithelial cell line was identified by immunofluorescence staining of actin cables that was confirmed by dual immunofluorescence co-localisation with phalloidin. Of 124 SMA-T positive sera, 123 reacted with the epithelial cell line and 120 with F-actin by ELISA, giving sensitivity for detection of anti-F-actin antibody of 99% and 97%, respectively. Of 64 SMA-V positive sera, four reacted with the epithelial cell line (6%) and 41 with F-actin by ELISA (64%). Tests of 493 normal blood donors and 100 disease controls yielded specificities of 584/593 (98.5%) and 562/593 (94.8%) for the cell line IFA and F-actin ELISA, respectively. The rat epithelial cell line IFA is a robust diagnostic assay for anti-F-actin antibody that can either replace the routine screening for actin-reactive SMA-T antibody in tissue sections or be used as...Continue Reading

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Citations

Nov 13, 2010·Journal of Gastroenterology·Ian R Mackay
Sep 23, 2014·Journal of Clinical Laboratory Analysis·Danilo VillaltaUNKNOWN Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy
Dec 31, 2011·Clinics and Research in Hepatology and Gastroenterology·Catherine Johanet, Eric Ballot
Aug 9, 2016·Clinical Reviews in Allergy & Immunology·Renato TozzoliNicola Bizzaro
Jul 12, 2017·Clinical & Translational Immunology·Ban-Hock Toh

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