PMID: 7544624Aug 23, 1995Paper

Actinobacillus actinomycetemcomitans leukotoxin forms large conductance, voltage-gated ion channels when incorporated into planar lipid bilayers

Biochimica Et Biophysica Acta
J D LearJ C Tanaka

Abstract

Actinobacillus actinomycetemcomitans leukotoxin is a member of the bacterial RTX (repeats in toxin) toxin family, produced by a diverse group of Gram-negative pathogens. Members of this group of toxins, although similar in sequence, differ in target cell specificity with Actinobacillus actinomycetemcomitans leukotoxin demonstrating a unique species- and cell-type specificity. Purified A. actinomycetemcomitans leukotoxin added to pre-formed POPE/POPS lipid bilayers showed no spontaneous incorporation (to concentrations of 250 ng/ml). Reproducible channel activity was seen when the bilayer was reformed from lipid monolayers in the presence of toxin (50 ng/ml) in one of the aqueous chambers. Control experiments with heat-inactivated toxin did not display channel activity under the same experimental conditions. The channel behavior showed a complex pattern of multiple conductance levels of 118, 262 and 406 pS in solutions containing 0.140 M NaCl. The first two states showed voltage-dependent channel gating with approximately equal but opposite apparent gating charges of 1.4 electrons. A model accounting for the multiple conducting states and gating properties is presented.

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Citations

Jul 10, 1999·Toxicon : Official Journal of the International Society on Toxinology·G P ZhangW Y Liu
Dec 26, 2001·Veterinary Microbiology·Sanjeev Kumar NarayananGeorge C Stewart
Jun 1, 1997·Trends in Microbiology·D H Meyer, P M Fives-Taylor
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Jun 10, 2010·FEMS Microbiology Reviews·Irena LinhartováPeter Sebo
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Mar 20, 2014·Infection and Immunity·Peter Svenssen MunksgaardHelle A Praetorius
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Feb 16, 2020·Molecular Oral Microbiology·Eric Krueger, Angela C Brown
Mar 1, 1996·Journal of Periodontology·Edward T LallyJacqueline C Tanaka
Jun 13, 1998·Biochimica Et Biophysica Acta·D KarakelianJ C Tanaka
Sep 2, 1999·Trends in Microbiology·E T LallyJ Korostoff
Dec 13, 1995·Biochimica Et Biophysica Acta·B Haimovich, J C Tanaka
Aug 26, 1998·Infection and Immunity·J KorostoffE T Lally

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