Actinobacillus pleuropneumoniae two-component system QseB/QseC regulates the transcription of PilM, an important determinant of bacterial adherence and virulence

Veterinary Microbiology
Jinlin LiuWeicheng Bei

Abstract

QseB/QseC is one of the five predicted two-component systems (TCSs) in Actinobacillus pleuropneumoniae. To understand the roles of this TCS in A. pleuropneumoniae, a markerless gene-deletion mutant ΔqseBC was constructed. Differentially expressed (DE) genes in ΔqseBC were filtered by microarray analysis. A total of 44 DE genes were found to be regulated by QseB/QseC system. The transcriptional profile of A. pleuropneumoniae ΔqseBC was compared with that of ΔluxS and catecholamine (CA) stimulations, 13 genes regulated by QseB/QseC were found also regulated by LuxS, and 3 Qse-regulons were co-regulated by CA stimulations, respectively. Binding of QseB to the promoters of three regulons (pilM, glpK and hugZ), which were co-regulated by QseB/QseC and LuxS, was evaluated by electrophoretic mobility-shift assay. Results indicated that pilM was directly regulated by phosphorylated-QseB. Then the pilM deletion mutant ΔpilM was constructed and characterized. Data presented here revealed that adherence ability of ΔpilM to St. Jude porcine lung cells was significantly decreased, and ΔpilM exhibited reduced virulence in pigs, suggesting PilM contributes to the process of A. pleuropneumoniae infection.

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Citations

Jun 24, 2017·FEMS Microbiology Letters·Chengkun ZhengWeicheng Bei
Jun 10, 2017·Genome Announcements·Zhenhua JiaShuishan Song
Apr 18, 2018·Frontiers in Cellular and Infection Microbiology·Huan LiWeicheng Bei
Oct 24, 2020·Pathogens and Disease·Josicelli Souza CrispimDenise Mara Soares Bazzolli
May 31, 2021·Veterinary Microbiology·Wei PengWeicheng Bei

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