Actinomycin D-deoxynucleotide complexes as models for the actinomycin D-DNA complex. The use of nuclear magnetic resonance to determine the stoichiometry and the geometry of the complexes.

Biochemistry
T R Krugh, Y C Chen

Abstract

The use of proton and carbon-13 magnetic resonance spectroscopy for the determination of the geometry and the stoichiometry of the actinomycin D-deoxyguanosine 5'-monophosphate complex is outlined. The dimerization of actinomycin D has been reexamined by recording the proton magnetic resonance spectrum of actinomycin D to much lower concentrations through the use of Fourier transform nuclear magnetic resonance techniques. The effect of the actinomycin D dimerization on the observed chemical shifts that results from the additon of nucleotides to an actinomycin D solution is directly demonstrated by comparing the actinomycin D-nucleotide titrations at both low (approximately 0.3 mM) and high (approximately 12 mM) concentrations of actinomycin D. In the presence of excess nucleotide the chemical shifts of the actinomycin D groups were essentially the same for both the low and high concentration titrations. The complexes of actinomycin D with pdG-dC, dG-dC, deoxyguanosine 3'-monophosphate, G-C, C-G, dIMP(5'), 2, 6-diaminopurine deoxyribose, and other nucleotides were also investigated by proton magnetic resonance and visible spectral titrations. These data were interpreted in terms of the molecular geometry of the complexes and in ...Continue Reading

Citations

Dec 14, 1977·Biochimica Et Biophysica Acta·M W DavidsonW D Wilson
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H E Auer, T N Thompson
Oct 1, 1986·Journal of Biomolecular Structure & Dynamics·R HoussinJ P Henichart
Dec 1, 1989·Journal of Biomolecular Structure & Dynamics·M DelepierreB P Roques
Jan 1, 1984·CRC Critical Reviews in Biochemistry·S Neidle, Z Abraham
Jun 6, 2003·Journal of Biochemistry and Molecular Biology·Hoon Yoo, Randolph L Rill

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