PMID: 8955388Dec 1, 1996Paper

Action at a distance for negative control of transcription of the glpD gene encoding sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12

Journal of Bacteriology
B Yang, T J Larson

Abstract

Aerobic sn-glycerol 3-phosphate dehydrogenase is a cytoplasmic membrane-associated respiratory enzyme encoded by the glpD gene of Escherichia coli. The glpD operon is tightly controlled by cooperative binding of the glp repressor to tandem operators (O(D)1 and O(D)2) that cover the -10 promoter element and 30 bp downstream of the transcription start site. In this work, two additional operators were identified within the glpD structural gene at positions 568 to 587 (0(D)3) and 609 to 628 (0(D)4). The two internal operators bound the glp repressor in the presence or absence of the tandem operators (O(D)1 and O(D)2) in vitro, as shown by DNase I footprinting. To assess a potential regulatory role for the two internal operators in vivo, a glpD-lacZ transcriptional fusion containing all four operators was constructed. The response of this fusion to the glp repressor was compared with those of fusion constructs in which O(D)3 and O(D)4 were inactivated by either deletion or site-directed mutagenesis. It was found that the repression conferred by binding of the glp repressor to O(D)1 and O(D)2 was increased five- to sevenfold upon introduction of the internal operators. A regulatory role for HU was suggested when it was found that rep...Continue Reading

References

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Citations

Dec 23, 2015·Molecular Microbiology·A Vimala, R Harinarayanan
Jan 8, 2013·Journal of Bacteriology·Axel Cournac, Jacqueline Plumbridge
Jun 20, 2018·Journal of Bacteriology·Jonathan H MartinJulie A Maupin-Furlow
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S AdhyaT Aki
Jul 4, 2006·Journal of Bacteriology·Amy L SpoeringKim Lewis

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