Action mechanism and signal pathways of Psidium guajava L. aqueous extract in killing prostate cancer LNCaP cells

Nutrition and Cancer
Kuan-Chou ChenRobert Y Peng

Abstract

Aqueous extract of Psidium guajava L. budding leaves (PE) has been shown to possess anti-prostate cancer activity in a cell line model. We examined whether its bioactivity could be conserved either in the presence or the absence of synthetic androgen R1881. In both cases, PE was shown to inhibit LNCaP cell proliferation and down-regulate expressions of androgen receptor (AR) and prostate specific antigen (PSA). The cytotoxicity of PE was shown by enhanced LDH release in LNCaP cells. The flow cytometry analysis revealed cell cycle arrests at G(0)/G(1) phase with huge amount of apoptotic LNCaP cells after treatment with PE for 48 h in a dose-responsive manner, which was also confirmed by TUNEL assay. From the results of decreased Bcl-2/Bax ratio, inactivation of phosphor-Akt, activation of phosphor-p38, phospho-Erk1/phospho-Erk2, the molecular action mechanism of PE to induce apoptosis in LNCaP cells was elucidated. Compatible with the in vitro study findings, treatment with PE (1.5 mg/mouse/day) significantly diminished both the PSA serum levels and tumor size in a xenograft mouse tumor model. Conclusively, PE is a promising anti-androgen-sensative prostate cancer agent.

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Citations

Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jaqueline Moraes BazioliJoão Ernesto de Carvalho
Aug 26, 2020·Anatomy & Cell Biology·Kamonwan JongsomchaiSugunya Mahatheeranont
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Engy A MahrousFatema R Saber
Jul 23, 2021·Critical Reviews in Food Science and Nutrition·Sarah JamiesonAnupam Bishayee

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Methods Mentioned

BETA
protein assay
ELISA
Assay
Fluorescence Microscopy
electrophoresis
Xenograft
enzyme linked immunoabsorbent assay
xenografts

Software Mentioned

CellQuest
ImagePro Plus

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