PMID: 2094820Sep 18, 1990Paper

Action of baclofen, GABA and antagonists on the membrane potential of cultured astrocytes of rat spinal cord

Neuroscience Letters
L HösliH Schramek

Abstract

The action of gamma-aminobutyric acid A (GABAA) and B (GABAB)-agonists has been studied on the membrane potential of astrocytes in explant cultures of rat spinal cord by means of intracellular microelectrode recordings. The GABAB-agonists (-)-baclofen and CGP 27 492 (3-aminopropyl phosphonous acid; 10(-6) to 10(-4) M) caused a hyperpolarization of the majority of astrocytes studied. On approximately 25% of the cells, the compounds had no effect. The hyperpolarization by baclofen (10(-4) M) was reversibly antagonized by the GABAB-antagonist 5-hydroxysaclofen (10(-4) M). GABA and the GABAA-agonist muscimol (10(-4) and 10(-3) M) depolarized approximately two thirds of the glial cells tested, whereas the remaining third remained unaffected. The GABAA-antagonist bicuculline (10(-4) and 10(-3) M) only reduced the depolarization by GABA (10(-4) M) but did not completely block it. On half of the cells tested, the depolarization by GABA was not affected by bicuculline, suggesting that the glial GABAA-receptor is different from the neuronal GABAA-receptor. Our electrophysiological investigations together with recent autoradiographic binding studies strongly suggest the existence of GABAB-receptors on astrocytes whereas there is less evid...Continue Reading

References

Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Bormann, H Kettenmann

❮ Previous
Next ❯

Citations

Apr 1, 1993·Progress in Neurobiology·E Hösli, L Hösli
Jun 26, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E HösliL Hösli
Jun 16, 2012·Neurochemical Research·Bo-Eun YoonC Justin Lee
Apr 29, 2006·Brain Research·Narito TateishiHiroyuki Ohno
Feb 2, 2018·Journal of Computational Neuroscience·Osamu HoshinoKazuo Watanabe
Oct 22, 1994·Proceedings. Biological Sciences·A M Butt, J Jennings
Jun 1, 1994·Glia·D D FraserB A MacVicar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.