PMID: 8963390Dec 1, 1995Paper

Action of endothelins on hepatic stellate cells

Journal of Gastroenterology
Norifumi KawadaK Decker

Abstract

To elucidate the role played by hepatic sinusoidal cells in the regulation of the circulatory status in the liver, the effect of endothelins (ETs) on primary-cultured stellate cells was examined. Kinetic analysis with 125I-labeled ET-1 revealed that stellate cells have ET receptors with a Kd value of 141 pM and a Bmax of 12.3 fmol/10(5) cells. ET-1, -2, and -3 dose-dependently increased inositol monophosphate (InsP) levels in stellate cells with an EC50 of 0.53, 1.63, and 1.88nM, respectively. Binding of 125I-labeled ET-1 to stellate cells and the ET-enhanced InsP formation were suppressed by preincubating the cells with 10 nM of unlabeled ET-1 or ET-3 for more than 3 h, indicating down-regulation and desensitization of ET receptors by homologous ligands. Binding of ETs to surface receptors induced a marked contraction of stellate cells. Stellate cells rapidly reacted to ETs, as detected by the flexible silicone-rubber-membrane method; 78%, 73%, and 58% of the stellate cells contracted 2.5 min after the addition of 10 nM of ET-1, ET-2, or ET-3, respectively. On the other hand, ETs also triggered a long-lasting contraction of the cells, as revealed with hydrated collagen gels. The ET-induced contraction of stellate cells decreas...Continue Reading

References

Jul 15, 1992·The Biochemical Journal·N KawadaK Decker
Sep 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D C RockeyS L Friedman
Jan 1, 1991·Journal of Cardiovascular Pharmacology·S FuruyaK Nokihara
Jan 1, 1991·The Journal of Clinical Investigation·C Serradeil-Le GalS Lotersztajn
Sep 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G M Rubanyi, L H Botelho
Mar 25, 1988·Biochimica Et Biophysica Acta·J KuiperT J van Berkel
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S L FriedmanD M Bissell
Jul 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y YokoiK Usui
Nov 30, 1984·Biochemical and Biophysical Research Communications·S Kawamoto, H Hidaka
Jan 1, 1981·Annual Review of Physiology·I H Pastan, M C Willingham
Oct 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M SakamotoK Tanikawa
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C HoussetD M Bissell
Mar 8, 1993·FEBS Letters·T A Tran-ThiK Decker

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Citations

Dec 2, 1998·Journal of Hepatology·E Olaso, S L Friedman
Aug 10, 1999·Journal of Gastroenterology and Hepatology·D Li, S L Friedman
Aug 26, 2011·World Journal of Hepatology·Cathy C MooreLaura W Schrum
Nov 6, 2008·Clinics in Liver Disease·Russell K Soon, Hal F Yee
Feb 17, 2005·European Journal of Pharmacology·Hiroko Matsui, Norifumi Kawada
Jul 13, 2004·Journal of Hepatology·Kusum K KharbandaDean J Tuma
Mar 25, 2016·International Journal of Molecular Sciences·Yujie Zhang, Branko Stefanovic
Jun 3, 2021·International Journal of Molecular Sciences·Andrés TabernillaMathieu Vinken

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