PMID: 6159645Sep 1, 1980Paper

Action of granulocyte-macrophage colony-stimulating factors: studies using a human leukemia cell line

Proceedings of the National Academy of Sciences of the United States of America
A J Lusis, H P Koeffler

Abstract

Granulocyte-macrophage colony-stimulating factors (CSFs) have previously been shown to stimulate colony formation in soft agar culture by a human myelogenous leukemia cell line known as KG-1. We have used KG-1 cells as a model system to investigate the interaction of CSF with myeloid cells. We now report that exposure of KG-1 cells to human CSFs in liquid culture results in a rapid (within 3 hr) burst of RNA synthesis and, after a lag of about 10 hr, a stimulation of DNA and protein synthesis. RNA and protein synthesis were maximally stimulated about 2-fold and DNA synthesis was stimulated about 2.5-fold. The stimulation was specific; various growth factors, hormones, and mouse CSFs had no effect on KG-1 macromolecular synthesis. Treatment with CSF did not discernibly alter the morphological appearance of the KG-1 cells (primarily myeloblasts) nor did it qualitatively affect the pattern of newly synthesized proteins separable by one- and two-dimensional electrophoresis. Several myeloid leukemia cell lines that were not responsive to CSF in agar culture, including a dedifferentiated variant of KG-1, showed little or no stimulation of macromolecular synthesis upon exposure to CSF. We have used the CSF-dependent stimulation of mac...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·F R MaxfieldI Pastan
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E R Stanley
Jan 1, 1978·Annual Review of Biochemistry·P A Marks, R A Rifkind
Jun 1, 1966·The Australian Journal of Experimental Biology and Medical Science·T R Bradley, D Metcalf
Mar 25, 1974·Biochemical and Biophysical Research Communications·S C ChangE Goldwasser
Nov 1, 1972·The Journal of Clinical Investigation·D W Golde, M J Cline
Dec 1, 1971·The Australian Journal of Experimental Biology and Medical Science·T R BradleyM A Sumner

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Citations

Mar 15, 1984·Klinische Wochenschrift·J Drews
Nov 1, 1988·International Journal of Cell Cloning·S C Clark
Nov 1, 1986·British Journal of Haematology·K Rhyner, R Taetle
Jan 1, 1983·Journal of Cellular Biochemistry·F F Wang, E Goldwasser
Jan 1, 1985·Critical Reviews in Oncology/hematology·R Taetle, J A Koziol

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