Action of heparin and ruthenium red on responses of reversibly-permeabilised rat mesenteric arteries

European Journal of Pharmacology
R S Garcha, A D Hughes

Abstract

Heparin and ruthenium red were introduced intracellularly into rat mesenteric resistance arteries via reversible-permeabilisation. Heparin and ruthenium red inhibited contractile responses to noradrenaline, but not caffeine in Ca(2+)-free conditions. Neither heparin nor ruthenium red significantly inhibited peak contractile responses to K+, noradrenaline or caffeine in physiological saline, although heparin significantly increased the time taken for peak force to develop in response to noradrenaline. Noradrenaline and calcium concentration-response relationships were unaffected by heparin. Experiments with permeabilised, fura-2 loaded vessels indicated that heparin inhibited Ca2+ release induced by noradrenaline, but did not inhibit caffeine-induced Ca2+ release. The peak rise in intracellular Ca2+ following K+, or noradrenaline in physiological saline was unaffected by heparin. The use of reversible permeabilisation may prove a useful approach, allowing introduction of a variety of membrane-impermeant blockers of second messenger systems into intact resistance arteries.

References

Jan 1, 1977·Physiological Reviews·M Endo
Nov 1, 1977·The Journal of Physiology·C Van Breemen
Feb 1, 1977·Circulation Research·A Fabiato, F Fabiato
Apr 1, 1977·The Journal of General Physiology·R Deth, R Casteels
Mar 1, 1992·Naunyn-Schmiedeberg's Archives of Pharmacology·M A Noguera, M P D'Ocon
Dec 31, 1987·Biochemical and Biophysical Research Communications·T D HillA L Boynton
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A V SomlyoA Scarpa
Apr 15, 1988·Biochemical and Biophysical Research Communications·M IinoM Endo
Apr 4, 1974·Pflügers Archiv : European journal of physiology·R Deth, C van Breemen
Jun 1, 1973·Circulation Research·D F Bohr
Jan 1, 1982·Journal of Cardiovascular Pharmacology·C CauvinC van Breemen
Jan 1, 1982·The Journal of Membrane Biology·H Miyamoto, E Racker

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Citations

Sep 23, 1997·British Journal of Pharmacology·R S Garcha, A D Hughes
Aug 28, 2001·British Journal of Pharmacology·R S GarchaA D Hughes
Aug 1, 1995·British Journal of Pharmacology·N A Parkinson, A D Hughes
May 20, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·M VadoriG Sava

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