PMID: 9430742Feb 12, 1998Paper

Action of immunoregulatory agents on 5-HETE production by cultured human amnion cells

Journal of Reproductive Immunology
S S EdwinD J Dudley

Abstract

Labor in women is associated with increased amniotic fluid prostaglandin (PG) concentrations. AF of women with term and preterm labor also contains elevated concentrations of arachidonic acid metabolites from the lipoxygenase pathway including 5-hydroxyeicosatetraenoic acid (5-HETE). We and others have shown that 5-HETE production within the uterus is increased by inflammatory cytokines. The purpose of this study was to determine whether 5-HETE production is regulated by immunotherapeutic agents that inhibit prostaglandin E2 (PGE2) production, such as interleukin-10 (IL-10) and cyclosporin A (CsA). Cultured confluent amnion cells were incubated for 16 hr with IL-10 (1 100 ng/ml), CsA (1 1000 ng/ml), or controls. Additional incubation of IL-10 and CsA (both at 10 and 100 ng/ml) were conducted on amnion cells in the presence and absence of interleukin-1 beta at 1 ng/ml. 5-HETE was measured by radioimmunoassay and cellular protein determined. IL-1 beta stimulated amnion cell 5-HETE production as expected. However, 5-HETE production by amnion cells was significantly inhibited by incubation with IL-10 and CsA, and both significantly attenuated 5-HETE production in response to IL-1 beta. We speculate that the inhibitory effect of IL-...Continue Reading

References

Aug 1, 1991·The Journal of Clinical Endocrinology and Metabolism·S Lundin-Schiller, M D Mitchell
Dec 1, 1989·The Journal of Experimental Medicine·D F FiorentinoT R Mosmann
Nov 1, 1989·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·E E SalamalekisP A Zourlas
May 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·R RomeroM D Mitchell
Dec 1, 1987·American Journal of Obstetrics and Gynecology·R RomeroM D Mitchell
Aug 1, 1988·Prostaglandins·R RomeroM D Mitchell
Dec 1, 1986·Australian and New Zealand Journal of Medicine·C J DugganJ F Mahony
Jul 1, 1994·American Journal of Obstetrics and Gynecology·K D HeyborneJ S Abrams
Dec 1, 1993·American Journal of Obstetrics and Gynecology·S S EdwinM D Mitchell
Sep 1, 1993·American Journal of Reproductive Immunology : AJRI·S S EdwinM D Mitchell
Mar 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·R RomeroM D Mitchell
Jun 1, 1996·Prostaglandins·S S EdwinM D Mitchell
Jun 1, 1997·Immunology Today·M Pretolani, M Goldman
Jun 1, 1997·Journal of Reproductive Immunology·D J DudleyM W Varner

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Citations

Dec 20, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Rachel J A HelliwellMurray D Mitchell
Sep 1, 2001·Obstetrics and Gynecology·D A TerroneW A Bennett
Feb 15, 2003·Medicinski pregled·Anita Mitreski, Gordana Radeka

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