PMID: 5904588Jan 1, 1966

Action of inhibitors of RNA and protein synthesis on cell enlargement

Plant Physiology
L D Noodén, K V Thimann

Abstract

Further studies with inhibitors of protein synthesis are presented to support the conclusion, drawn from work with chloramphenicol, that protein synthesis is a critical limiting factor in auxin-induced cell expansion. The indoleacetic acid-induced elongation of oat coleoptile sections was strongly inhibited by dl-p-fluorophenylalanine, and the inhibition is antagonized by phenylalanine. Puromycin at 10(-4)m very strongly inhibited the indoleacetic acid-induced growth of oat coleoptile and artichoke tuber sections and exerted a less powerful effect on pea stem sections. As found earlier with chloramphenicol, concentrations of puromycin effective in inhibiting the growth of coleoptile sections had quantitatively similar effects on protein synthesis, as measured by the incorporation of C(14)-leucine into protein of the coleoptile tissue. Several analogues of RNA bases were also tested, but while 8-azaguanine very strongly inhibited growth of artichoke tuber disks, 6-azauracil was the only one of this group clearly inhibitory to growth in coleoptile or pea stem sections. Actinomycin D actively inhibited both elongation and the incorporation of C(14)-leucine into protein in oat coleoptile sections. Inhibition of the 2 processes went...Continue Reading

References

Jan 1, 1965·Plant Physiology·L D Noodén, K V Thimann
Oct 14, 1964·Biochemical and Biophysical Research Communications·H Klenow, S Frederiksen
Mar 1, 1962·Experimental Cell Research·R E CERRONI, E ZEUTHEN
Jul 1, 1963·The Journal of Cell Biology·M L BIRNSTIEL, B B HYDE
Dec 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·M W KARAKASHIAN, J W HASTINGS
Aug 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·R E CLICK, D P HACKETT
Jan 1, 1963·Annual Review of Biochemistry·R W BROCKMAN, E P ANDERSON
Apr 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·D NATHANS

Citations

Jan 1, 1979·Planta·G W Bates, R Cleland
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L M SrivastavaI E Taylor
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L N Vanderhoef, C A Stahl
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A N Heyn
Aug 1, 1967·Plant Physiology·B D Patterson, A J Trewavas
Feb 1, 1969·Plant Physiology·W G Hopkins, K F Bonnell
May 1, 1976·Plant Physiology·L N VanderhoefK A Brinkmann
Aug 1, 1978·Journal of Steroid Biochemistry·R W Watts, J F Wheldrake
Nov 19, 1969·Biochimica Et Biophysica Acta·B Kessler, I Snir
Jan 1, 1974·Advances in Enzyme Regulation·A G Gornall, C C Liew
Aug 9, 1967·Annals of the New York Academy of Sciences·R M Klein
Aug 9, 1967·Annals of the New York Academy of Sciences·J L KeyC Y Lin
Jul 1, 1969·Archives of Biochemistry and Biophysics·A N Heyn

Related Concepts

Antimetabolites
Plant Cells

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