PMID: 6404307May 4, 1983Paper

Action of ions and pH on the binding of virginiamycin S to ribosomes

Biochimica Et Biophysica Acta
M Di Giambattista, C Cocito

Abstract

Virginiamycin S (VS) binds to the 50 S ribosomal subunits (KaVS = 2.5 X 10(6) M-1); the affinity of ribosomes for VS undergoes a 6-fold increase (KaVS = 15 X 10(6) M-1) in the presence of virginiamycin M (VM). In the present work, the action of inorganic ions and pH on the binding reaction of VS to ribosomes was analyzed by a spectrofluorimetric technique, in the presence and in the absence of VM. Preliminary to this study, the interaction of ions with free VS was also explored. In aqueous solution and in the absence of VM, chelation by VS of K+, NH4+, Na+, Mg2+ and Ca2+ was observed. The binding of Mg2+ was strongly influenced by monovalent ions and pH between 7 and 8: the association constants for the VS:Mg2+ complex were 12.5 M-1 (+NH4+) and 45 M-1 (-NH4+). Binding of VS to ribosomes occurred as well in the presence of NH4+ as with K+, but was suppressed upon replacement of these ions by Na+. The association constant of the VS binding reaction to ribosomes was strongly enhanced by VM in the presence of either NH4+ or K+ but, while a plateau value was observed over the entire range of NH4+ concentrations, 100-fold higher values were obtained at low (25 mM) K+. This VM-induced increase of ribosome affinity for VS (KaVS) was de...Continue Reading

References

Jun 1, 1978·Molecular & General Genetics : MGG·C Cocito
Oct 25, 1978·Molecular & General Genetics : MGG·R ParfaitC Cocito
Apr 15, 1977·European Journal of Biochemistry·A Contreras, D Vázquez
Feb 1, 1974·Archives of Biochemistry and Biophysics·H L Ennis
Jan 28, 1971·Journal of Molecular Biology·O P van DiggelenL Bosh
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Parfait, C Cocito
Jul 7, 2009·Archives of Physical Medicine and Rehabilitation·Michael I WeintraubSteven P Cole

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Citations

Jul 5, 2005·Expert Opinion on Investigational Drugs·D Beyer, K Pepper

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