Abstract
A loss of TNF receptors expression has been found in advanced lung cancers, and human A549 lung adenocarcinoma cells are resistant to the cytotoxic effects of TNF-alpha and cisplatin. Here, the mechanisms of the drug resistance of A549 were extensively studied by gene modulation of the cells by solamargine (SM) which was isolated from Solanum incanum herb. SM induced morphological changes of chromatin condensation, DNA fragmentation, and sub-G(1) peak in a DNA histogram of A549 cells, indicating cell death by apoptosis. SM elevated the expressions of TNF-R1 and -R2 and overcame the resistance of A549 cells to TNF-alpha and -beta. The recruitment of TRADD, FADD, and activation of caspase-8 and -3 in SM-treated A549 cells evidenced the activation of TNFRs signal transduction. In addition, release of cytochrome c from mitochondria, down-expression of Bcl-2 and Bcl-x(L), up-regulation of Bax, and caspase-9 activities were observed in SM-treated A549 cells. Combinational treatment of SM and cisplatin synergistically enhanced caspase-8, -9, and -3 activities in A549 cells. Thus, SM sensitizes A549 cells through TNFRs and mitochondria-mediated pathways and may have anticancer potential against TNFs- and cisplatin-resistance lung cance...Continue Reading
References
Sep 1, 1995·American Journal of Respiratory Cell and Molecular Biology·H ShimomotoK Shimokata
Jul 21, 1998·Cancer·T A TranJ S Ross
Feb 7, 2001·Biochemical and Biophysical Research Communications·Z DingS C Tang
Oct 20, 2000·Oncogene·Q ChengG Cheng
Dec 8, 2000·Biochemical Pharmacology·K W KuoH M Sheu
Apr 28, 2001·Science·M C WeiS J Korsmeyer
Sep 12, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·S S YoonJ Kim
Oct 5, 2001·Molecular Cell·E H ChengS J Korsmeyer
Oct 31, 2001·Helicobacter·J S KimI S Song
Nov 27, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·M Krzakowski
Jan 5, 2002·Cancer Gene Therapy·S SanliogluK W Thomas
Jan 25, 2002·International Journal of Cancer. Journal International Du Cancer·Ute A Sartorius, Peter H Krammer
Mar 5, 2002·Nature Medicine·Heidi LeBlancAvi Ashkenazi
Mar 19, 2002·Cellular Signalling·David J MacEwan
Mar 20, 2002·Current Molecular Medicine·S Gupta
Nov 16, 2002·Hearing Research·Prasad DevarajanFederico Kalinec
Mar 8, 2003·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·V M MaruoH S Spinosa
Mar 15, 2003·The Journal of Biological Chemistry·Abdellah MansouriFrançois X Claret
Apr 12, 2003·Leukemia Research·Masakazu SawanoboriMasanobu Kitagawa
Citations
Aug 28, 2010·Cancer Metastasis Reviews·Limin ShuAh-Ng Kong
Sep 22, 2007·Cell Biology and Toxicology·L Y ShiuK W Kuo
Oct 8, 2013·Bioorganic & Medicinal Chemistry Letters·Li-Hua MuPing Liu
Mar 15, 2006·Journal of Immunotherapy·Robbert G van der MostRichard A Lake
Aug 5, 2008·American Journal of Respiratory Cell and Molecular Biology·Jingmei ZhangDaya Upadhyay
Jul 2, 2011·Biological & Pharmaceutical Bulletin·Akemi HayakawaJunji Magae
Jun 27, 2013·Indian Journal of Pharmaceutical Sciences·D J SinghMala D Menon
Apr 15, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wen GaoYongqian Shu
May 23, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yan ZhouSwei Hann
May 27, 2011·Journal of Dermatological Science·Chin-Han WuHamm-Ming Sheu
Nov 21, 2007·Journal of Ethnopharmacology·Y B JiX Zou
Jul 10, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L Y ShiuK W Kuo
Jun 23, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Ooi Kheng LeongShaida Fariza Sulaiman
Mar 31, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Iman Karimi SaniFatemeh Kalalinia
Oct 29, 2011·Journal of Ethnopharmacology·Soo-Jin JeongSung-Hoon Kim
Feb 22, 2012·Biochimica Et Biophysica Acta·Chia-Hua LiangJyh-Horng Sheu
Dec 17, 2011·Journal of Ethnopharmacology·Xia DingSi-Guo Gao
Aug 24, 2010·Brazilian Journal of Biology = Revista Brasleira De Biologia·P M VieiraL Chen-Chen
Apr 7, 2017·Phytotherapy Research : PTR·Fatemeh Kalalinia, Iman Karimi-Sani
Nov 23, 2018·Biological & Pharmaceutical Bulletin·Li-Hua MuPing Liu
Jul 21, 2007·Molecular Nutrition & Food Research·Chia-Hua LiangKou-Wha Kuo
Aug 7, 2019·The Journal of Pharmacy and Pharmacology·Mariza A MirandaMaria V L B Bentley
Aug 14, 2008·Bioscience Reports·Li Yen ShiuKou Wha Kuo
May 27, 2005·World Journal of Gastroenterology : WJG·Kuo-Ching YangShyr-Yi Lin
Apr 11, 2017·Molecular Medicine Reports·Jing SunDong-Yan Guo
May 4, 2018·BMC Complementary and Alternative Medicine·Trevor BurgerWerner Cordier