PMID: 2507759Aug 1, 1989Paper

Action spectra for photoinduced inactivation of bacteriophage T7 sensitized by 8-methoxypsoralen and angelicin

Journal of Photochemistry and Photobiology. B, Biology
G RontóK Modos

Abstract

The action spectrum (240-300 nm) for photoinactivation of unsensitized phage T7 and the action spectra (310-380 nm) for photoinactivation of phage T7 sensitized with 8-methoxypsoralen (8-MOP) and angelicin were measured by an automated method. For unsensitized phage T7 the action spectrum is in good agreement with the absorption spectrum. For sensitization with angelicin the action spectrum is similar to the absorption spectrum, but for sensitization with 8-MOP the spectra are different. The agreement between the T7 absorption and action spectra in the far-UV region is due to photodamage of DNA, leading to phage inactivation. The similarity in the action and absorption spectra in the near-UV region for sensitization with angelicin seems to be in accordance with the monofunctional photobinding of angelicin to DNA. The action spectrum for sensitization with 8-MOP has a maximum at about 320 nm and this suggests that, in addition to the monoadducts, the biadducts play a role in the inactivation of phage T7. Taking the number of bound furocoumarin molecules into consideration, the quantum efficiencies were estimated. Furocoumarin increases the quantum efficiency in the near-UV region and the values are similar to those obtained in f...Continue Reading

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Citations

Apr 15, 1990·Journal of Photochemistry and Photobiology. B, Biology·K TóthG Rontó
Feb 28, 1992·Journal of Photochemistry and Photobiology. B, Biology·G RontóA Bérces
Jul 7, 1999·Journal of Photochemistry and Photobiology. B, Biology·K ModosG Ronto
Jan 7, 2014·Journal of Photochemistry and Photobiology. B, Biology·István-Zoltán VassImre Vass
Dec 24, 2013·Journal of Photochemistry and Photobiology. B, Biology·Emanuelle B F SilvaJosé P Siqueira-Júnior
Mar 29, 2014·Journal of Food and Drug Analysis·Ying-Sing Li, Jeffrey S Church
Jan 1, 1992·Journal of Photochemistry and Photobiology. B, Biology·G RontóG Csik

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