Actions of ethanolamine on cultured sensory neurones from neonatal rats

Neuroscience Letters
Hesham KhairyRoderick H Scott

Abstract

Some of the analgesic and antinociceptive properties of the endocannabinoid anandamide can be explained by modulation of voltage-activated ion channels. However, the products of anandamide metabolism by fatty acid amide hydroxylase may also contribute to the altered excitability of sensory neurones. Ethanolamine is a product of metabolism of acylethanolamines including anandamide. In this study whole cell patch clamp recording and fura-2 Ca(2+) imaging techniques were used to characterize its actions on neonatal rat cultured dorsal root ganglion neurones. Ethanolamine (1muM) increased the mean Ca(2+) transient produced by 1mM caffeine and modulated Ca(2+) transients evoked by 60mM KCl. Thapsigargicin (500nM) inhibited the ethanolamine-evoked enhancement of Ca(2+) transients evoked by depolarisation. Voltage-activated K(+) currents were evoked from a holding potential of -70mV by voltage step commands to 0mV. Acute application of 1muM ethanolamine produced irreversible current modulation. However, application of 100nM ethanolamine reversibly increased or decreased K(+) currents. These effects of ethanolamine on voltage-activated K(+) currents were not sensitive to continual application of thapsigargicin. When applied alone thaps...Continue Reading

References

Jan 12, 2002·Current Opinion in Pharmacology·Leslie Iversen, Victoria Chapman
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May 1, 2008·Nature Reviews. Drug Discovery·Vincenzo Di Marzo
Mar 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Isobel J LeverAndrew S C Rice

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