Actions of taurine on the L-type Ca2+ channel current in guinea pig ventricular cardiomyocytes

Journal of Cardiovascular Pharmacology
H Satoh, M Horie

Abstract

Effects of taurine on the L-type channel in isolated guinea pig ventricular cardiomyocytes were examined at different Ca2+ concentrations by using whole-cell and cell-attached voltage-clamp modes. All experiments were performed at 36 degrees C. In whole-cell voltage-clamp experiments, test pulses were applied between -20 to +60 mV from a holding potential of -40 mV. When [Ca]i was pCa 6, addition of 10 and 20 mM taurine to the bath solution reduced the Ca2+ current (I(Ca)) at 0 mV by 14.4 +/- 2.0% (n = 8; p < 0.01) and 31.5 +/- 2.2% (n = 8; p < 0.001), respectively. In contrast, when [Ca]i was pCa 8, I(Ca) at +10 mV was enhanced by 10.1 +/- 2.2% (n = 7; p < 0.05) at 10 mM taurine and by 41.7 +/- 2.1% (n = 7; p < 0.001) at 20 mM taurine. Taurine increased the time constants (tau(f) and tau(s)) of inactivation phase for I(Ca) current at both pCa 8 and 6. In cell-attached voltage-clamp experiments, taurine (20 mM) decreased the open probability of unitary Ba2+ current from 0.63 +/- 0.06 to 0.39 +/- 0.09 (n = 5; p < 0.01) at 5.4 mM [Ca]o, whereas taurine increased it from 0.21 +/- 0.04 to 0.48 +/- 0.07 (n = 4; p < 0.01) at 0.9 mM [Ca]o. Taurine did not affect the channel conductance. In addition, taurine (20 mM) increased the time ...Continue Reading

References

Jul 21, 1992·European Journal of Pharmacology·H Satoh, N Sperelakis
Apr 1, 1990·Cell Calcium·A SawamuraN Sperelakis
Jan 1, 1989·Progress in Biophysics and Molecular Biology·E Carbone, D Swandulla
May 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·H Satoh, K Hashimoto
Jan 1, 1984·Progress in Biophysics and Molecular Biology·R Eckert, J E Chad
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Sep 1, 1983·Pflügers Archiv : European journal of physiology·A CavaliéW Trautwein
Sep 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·J B Lombardini, D L Bricker
Oct 15, 1982·Biochemical Pharmacology·F FranconiA Giotti
Jul 1, 1980·Biochimica Et Biophysica Acta·R Y Tsien, T J Rink
Jan 1, 1995·Journal of Cardiovascular Pharmacology·H Satoh
Feb 16, 1993·European Journal of Pharmacology·H Satoh, N Sperelakis
Jun 1, 1996·Japanese Journal of Pharmacology·H SadaN Sperelakis

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Citations

Apr 2, 1998·General Pharmacology·H Satoh, N Sperelakis
Mar 2, 1999·British Journal of Pharmacology·H Satoh
Oct 18, 2007·Acta Haematologica·Maya Otto-DuesselJohn C Wood
Sep 1, 2012·Amino Acids·Stephen W SchafferJunichi Azuma
May 23, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Denis V Abramochkin, Matti Vornanen

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