Actions of the Cl- channel blocker NPPB on absorptive and secretory transport processes of Na+ and Cl- in rat descending colon.

Acta Physiologica Scandinavica
M Diener, W Rummel

Abstract

The effects of the Cl- channel blocker, NPPB (5-nitro-2-(3-phenylpropylamino)-benzoate), on the transport of Na+ and Cl- in the descending colon of the rat were studied in the Ussing chamber. In control tissue, NPPB administered at the mucosal side of the epithelium increased the short-circuit current (Isc) and inhibited the unidirectional mucosa-to-serosa fluxes of Na+ and Cl-. In HCO3- - or Cl- -free media for in the presence of SITS (4-acetamido-4'-isothiocyanato-stilbene-2,2'-disulphonic acid), this increase in Isc caused by mucosal NPPB was not observed. The serosal administration of NPPB was without effect. Mucosal NPPB (10(-4) mol l-1) decreased the forskolin-induced increase in Isc by only about 60%. However, the activation of the serosa-to-mucosa flux of Cl- caused by forskolin was inhibited completely. NPPB decreased the mucosa-to-serosa fluxes of Na+ and Cl- reduced additionally by forskolin. Serosal NPPB decreased Isc and FNasm, but had no effect on FNams or FClmas. In HCO3- -free buffer the increase in Isc induced by forskolin was inhibited completely by NPPB. The inhibition of Cl- secretion by NPPB fits well with the capacity of the drug to block Cl- channels. For the inhibition of neutral NaCl absorption two site...Continue Reading

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Citations

Sep 1, 1991·Pflügers Archiv : European journal of physiology·M BöhmeW Rummel
Sep 1, 1991·Pflügers Archiv : European journal of physiology·R GregerH J Lang
Jan 1, 1994·Pflügers Archiv : European journal of physiology·M HatchN D Vaziri
Sep 1, 1992·Pflügers Archiv : European journal of physiology·M DienerW Rummel
Jul 27, 2006·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·E-M BleichG Breves
Mar 13, 1990·European Journal of Pharmacology·M Diener, W Rummel
Aug 14, 1998·General Pharmacology·G Schultheiss, M Diener
Apr 12, 2001·Journal of Neurochemistry·A JeremicS Jeftinija
Jul 1, 1996·British Journal of Pharmacology·M DienerE Scharrer
Jul 1, 1992·Acta Physiologica Scandinavica·M Hemlin, A Mellander
May 1, 1994·Acta Physiologica Scandinavica·D VeronE E Arrizurieta
Aug 16, 2002·The Journal of Physiology·Hideki SakaiNoriaki Takeguchi
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Aug 24, 1990·Biochimica Et Biophysica Acta·H Gögelein, K Capek
Jul 26, 2016·Frontiers in Physiology·Sabine SchindeleMartin Diener
Nov 20, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Julia Steidle, Martin Diener
Dec 9, 1998·The American Journal of Physiology·S HörgerM Diener

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