Actions of the Kunitz-type serine protease inhibitor Amblyomin-X on VEGF-A-induced angiogenesis

Toxicon : Official Journal of the International Society on Toxinology
C C DrewesS H P Farsky

Abstract

Amblyomin-X is a Kunitz-type serine protease inhibitor (Kunitz-type SPI) designed from the cDNA library of the Amblyomma cajennense tick, which displays in vivo anti-tumor activities. Here, the mechanisms of actions of Amblyomin-X in vascular endothelial growth factor A (VEGF-A)-induced angiogenesis were characterized. Topical application of Amblyomin-X (10 or 100 ng/10 μl; each 48 h) inhibited VEGF-A-induced (10 ng/10 μl; each 48 h) angiogenesis in the dorsal subcutaneous tissue in male Swiss mice. Moreover, similar effect was observed in the VEGF-A-induced angiogenesis in the chicken chorioallantoic membrane (CAM). Additional in vitro assays in t-End cells showed that Amblyomin-X treatment delayed the cell cycle, by maintaining them in G0/G1 phase, and inhibited cell proliferation and adhesion, tube formation and membrane expression of the adhesion molecule platelet-endothelial cell adhesion molecule-1 (PECAM-1), regardless of mRNA synthesis. Together, results herein reveal the role of Kunitz-type SPI on in vivo VEGF-A-induced angiogenesis, by exerting modulatory actions on endothelial cell proliferation and adhesion, especially on membrane expression of PECAM-1. These data provide further mechanisms of actions of Kunitz-type...Continue Reading

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Citations

Feb 26, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Janaína Souza VenturaAna Marisa Chudzinski-Tavassi
Jan 23, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ana Marisa Chudzinski-TavassiJean Gabriel de Souza
Jun 17, 2015·Toxicon : Official Journal of the International Society on Toxinology·Ana Carolina Prado SousaMarcelo José Barbosa Silva
Aug 15, 2015·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Francielle A CordeiroEliane C Arantes
Apr 29, 2015·Toxicon : Official Journal of the International Society on Toxinology·C C DrewesS H P Farsky
Nov 8, 2017·Medical and Veterinary Entomology·L F PariziI DA Silva Vaz
Aug 28, 2016·Oncotarget·Jean Gabriel de SouzaAna Marisa Chudzinski-Tavassi
Jul 25, 2019·Frontiers in Physiology·Iveta ŠtibrániováMária Kazimírová
Mar 7, 2020·Frontiers in Cellular and Infection Microbiology·Charles E HartSaravanan Thangamani
Jun 8, 2017·Frontiers in Cellular and Infection Microbiology·Adrien A BlisnickSarah I Bonnet
Jan 23, 2021·International Journal of Molecular Sciences·Mohamed Amine JmelMichalis Kotsyfakis

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