Actions of thermal stress in two-cell bovine embryos: oxygen metabolism, glutathione and ATP content, and the time-course of development

Reproduction : the Official Journal of the Society for the Study of Fertility
R M RiveraPeter J Hansen

Abstract

The mechanism by which heat shock disrupts development of the two-cell bovine embryo was examined. The reduction in the proportion of embryos that became blastocysts caused by heat shock was not exacerbated when embryos were cultured in air (20.95% O(2)) as compared with 5% O(2). In addition, heat shock did not reduce embryonic content of glutathione, cause a significant alteration in oxygen consumption, or change embryonic ATP content. When embryos were heat-shocked at the two-cell stage and allowed to continue development until 72 h post insemination, heat-shocked embryos had fewer total nuclei and a higher percentage of them were condensed. Moreover, embryos became blocked in development at the eight-cell stage. The lack of effect of the oxygen environment on the survival of embryos exposed to heat shock, as well as the unchanged content of glutathione, suggest that free radical production is not a major cause for the inhibition in development caused by heat shock at the two-cell stage. In addition, heat shock appears to have no immediate effect on oxidative phosphorylation since no differences in ATP content were observed. Finally, the finding that heat shock causes a block to development at the eight-cell stage implies tha...Continue Reading

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Citations

Feb 9, 2005·The Journal of Reproduction and Development·Takaya MatsuzukaYukio Kanai
Sep 3, 2008·The Journal of Reproduction and Development·Miki SakataniMasashi Takahashi
Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Z Roth, P J Hansen
Jan 20, 2016·International Journal of Biometeorology·Syma Ashraf WaizR C Upadhyay
Jun 18, 2010·Biology of Reproduction·Csaba PribenszkyJohn Yovich
Jun 16, 2018·PloS One·Łukasz RąpałaAnna M Duszewska
Jun 30, 2018·Molecular Reproduction and Development·Flavia R O de Barros, Fabíola F Paula-Lopes
Feb 16, 2019·Molecular Reproduction and Development·Raffaele Boni

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