Activatable fluorescence imaging of macrophages in atherosclerotic plaques using iron oxide nanoparticles conjugated with indocyanine green

Atherosclerosis
Hiroyuki IkedaSusumu Miyamoto

Abstract

Macrophages are key factors in the formation of unstable atherosclerotic plaques, which may be identified through macrophage imaging. We tested whether activatable fluorescence probes of iron oxide nanoparticles (IONPs) conjugated with indocyanine green (ICG) (IONP-ICG), consisting of biocompatible reagents, can visualize macrophages present in atherosclerotic plaques. IONP-based probes conjugated with different numbers of ICG molecules were synthesized. Six-week-old spontaneously hyperlipidemic (SHL) mice were fed either a Western or normal diet for 14 weeks, and were intravenously injected with IONP-ICG (55.8 mg Fe/kg). Aortas were harvested 48 h later, and aortas containing atherosclerotic plaques were imaged. Phantom imaging studies using IONP-ICG solution demonstrated that the addition of surfactants to IONP-ICG solutions yielded fluorescence activation. Incubation of macrophages with IONP-ICG led to internalization of IONP-ICG and near infrared fluorescence (NIRF) activation. In NIRF imaging studies, intense fluorescence signals were clearly visible primarily at the margins of atherosclerotic plaques, and relatively weak signals were evident inside the plaques, demonstrating the feasibility of detection of NIRF signals at...Continue Reading

Citations

Nov 21, 2019·Nanomaterials·Marina S DukhinovaVladimir V Vinogradov
Jan 2, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Gurneet S SanghaAlisa M Clyne
Apr 4, 2021·Journal of Biomedical Materials Research. Part a·Yana G ToropovaKamil G Gareev
Nov 11, 2020·Nanomedicine : Nanotechnology, Biology, and Medicine·Menglin WuXuening Zhang
Jun 8, 2021·Frontiers in Cardiovascular Medicine·Zhaoyue LiYingfeng Tu

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