Activated coagulation factor X: a novel mitogenic stimulus for human mesangial cells.

Journal of the American Society of Nephrology : JASN
Raffaella MonnoFrancesco P Schena

Abstract

Intraglomerular activation of the coagulation cascade is a common feature of mesangioproliferative glomerulonephritis. Besides thrombin, very little is known about the cellular effects of other components of the coagulation system. This study investigated the effect of activated factor X (FXa) on cultured human mesangial cells. This serine protease induced a significant and dose-dependent increase in DNA synthesis. In addition to its mitogenic effect, FXa caused a striking upregulation of platelet-derived growth factor (PDGF) A and B chain gene expression. Next, the intracellular mitogenic signaling pathways activated by FXa were investigated. FXa induced a rapid spike in cytosolic calcium concentration followed by a sustained plateau. This response was not influenced by the downregulation of thrombin receptors. In addition, FXa stimulated a significant upregulation of different tyrosine-phosphorylated proteins. One of these phosphorylated cellular proteins was represented by the c-jun N-terminal kinase, a member of the mitogen-activated protein kinase family. To evaluate the role of FXa enzymatic activity and of PDGF autocrine secretion, FXa-induced DNA synthesis was studied in the presence of leupeptin, a specific serine prot...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·J Pouysségur, K Seuwen
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G P GasicG J Gasic
Mar 1, 1992·Journal of Cellular Physiology·C J HeE Rondeau
Dec 1, 1990·Molecular and Cellular Biology·W R HuckleS Earp
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H IidaD F Bowen-Pope
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S ParthasarathyD Steinberg
Sep 1, 1989·The American Journal of Physiology·P J ShultzH E Abboud
Oct 1, 1988·The American Journal of Physiology·P J ShultzH E Abboud
Jul 1, 1987·Kidney International·T TakemuraS Maki
Aug 1, 1986·The Journal of Cell Biology·C GajdusekD Stern
Oct 1, 1972·Kidney International·P Kincaid-Smith
Oct 1, 1994·The American Journal of Physiology·G GrandalianoH E Abboud
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·S NystedtJ Sundelin
Oct 1, 1994·The American Journal of Physiology·M GranoA Teti
May 1, 1994·The Journal of Experimental Medicine·G GrandalianoH E Abboud
Sep 1, 1993·Kidney International·G GrandalianoH E Abboud
Jan 15, 1996·The Biochemical Journal·R J GrandP W Grabham
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·E M GordonF L Hall
Oct 4, 1996·The Journal of Biological Chemistry·J M Kyriakis, J Avruch
Apr 29, 1998·Journal of the American Society of Nephrology : JASN·G GrandalianoH E Abboud
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W F XuD C Foster
Jan 20, 1999·Experimental Nephrology·G GrandalianoF P Schena

❮ Previous
Next ❯

Citations

Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Giuseppe GrandalianoFrancesco P Schena
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Ortega-VelázquezD Rodríguez Puyol
Jun 23, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·María P Ruiz-TorresMaria L Díez-Marqués
Dec 21, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Keiko NomuraTakahiko Ono
Jun 5, 2007·Orvosi hetilap·Krisztina FischerAndrás Szabó
Sep 9, 2008·Trends in Molecular Medicine·Keren BorensztajnC Arnold Spek
Dec 9, 2009·Journal of Cellular and Molecular Medicine·Keren BorensztajnC Arnold Spek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Coagulation Signaling Pathways

Coagulation is the process by which a blood clot is formed. This process includes both the formation of a platelet plug as well as a cascade of clotting factors resulting in the formation of fibrin strands. Find the latest research on coagulation signaling pathways here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.