Activated eosinophils stimulate endothelin-1 release from airway epithelial cells by direct adherence via adhesion molecules

Pulmonary Pharmacology & Therapeutics
T EndoS Hasegawa

Abstract

Endothelin-1 (ET-1), synthesized in airway epithelial cells, has a potent constrictive action on airway smooth muscle. In this study, we investigated the effect of eosinophils on ET-1 release from guinea pig cultured tracheal epithelial cells. Eosinophils with or without the activation were directly co-cultured with tracheal epithelial cells. Eosinophils activated by GM/CSF or IL-5 potentiated ET-1 release, but non-activated ones did not. Treatment of activated eosinophils with antibodies against macrophage-1 (Mac-1) and/or very late antigen-4 (VLA-4) suppressed the potentiated ET-1 release. However, inhibition of lipid mediators derived from activated eosinophils could not suppress the potentiated ET-1 release. Moreover, separated co-culture of activated eosinophils with tracheal epithelial cells using Millicell-CM had no effect on ET-1 release. These observations suggest that adherence of activated eosinophils to epithelial cells via adhesion molecules such as Mac-1 and VLA-4 was essential for potentiation of ET-1 release. Since presence of eosinophils in the epithelial layer has been commonly demonstrated in bronchial biopsies and autopsy specimens from patients with asthma, epithelial cells would be activated by adherence o...Continue Reading

Citations

Dec 28, 1999·Respirology : Official Journal of the Asian Pacific Society of Respirology·J Xu, N S Zhong
Dec 6, 2001·American Journal of Respiratory and Critical Care Medicine·D J Tschumperlin, J M Drazen
Jul 29, 2006·Expert Opinion on Pharmacotherapy·Henry Milgrom
Aug 13, 2009·Current Allergy and Asthma Reports·Bill T Ameredes, William J Calhoun
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Peter J HenryRoy G Goldie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.