PMID: 7522324Sep 13, 1994Paper

Activated Ki-Ras complements erythropoietin signaling in CTLL-2 cells, inducing tyrosine phosphorylation of a 160-kDa protein

Proceedings of the National Academy of Sciences of the United States of America
Y YamamuraY Ikawa

Abstract

We have previously shown that expression of erythropoietin (EPO) receptor (EPOR) alone failed to confer EPO responsiveness on the interleukin 2-dependent T-cell line CTLL-2, whereas the introduction of the EPOR into interleukin 3-dependent pro-B-cell lines, such as BAF-B03, allowed the cells to proliferate in response to EPO. Here, we report that additional expression of v-Ki-Ras conferred EPO-dependent growth on CTLL-2 cells expressing the EPOR, with additional formation of a high-affinity EPOR. To investigate possible mechanisms of EPOR downstream signaling induced by v-Ki-Ras expression in these CTLL-2-derived cells, we carried out anti-phosphotyrosine immunoblot analysis of the EPOR complex immunoprecipitated with anti-EPOR antibody from lysates of cells with and without cytokine stimulation, revealing two 160-kDa and 130-kDa phosphotyrosyl proteins. An anti-JAK2 antibody did not react with these proteins, suggesting that they may represent cellular components involved in an EPO-EPOR signaling pathway induced by v-Ki-Ras. Similar phosphotyrosyl proteins were present among Friend erythroleukemia cell lines, in which the Friend virus gp55/EPOR complex on the cell surface constitutively sends signals for cell growth.

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D LinnekinW L Farrar
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D BowtellP Senior
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D E Quelle, D M Wojchowski
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Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J F Bazan
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Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K TodokoroY Ikawa
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M NodaR H Bassin

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Citations

Mar 23, 2001·Journal of Toxicology and Environmental Health. Part a·E A KönigZ L Marcsek
Jan 1, 1996·Annual Review of Cell and Developmental Biology·S S WatowichH F Lodish

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