Nov 22, 2011

Activated lymphocytes and high liver expression of IFN-γ are associated with fulminant hepatic failure in patients

Liver International : Official Journal of the International Association for the Study of the Liver
Damião C M dos SantosMarcelo Alves Pinto

Abstract

To study immunological mechanisms of fulminant hepatic failure (FHF) derived from extensive liver lesions, 14 patients with FHF induced by different aetiologies were investigated by observance of both lymphocyte phenotyping and cytokine levels. Five patients bearing benign acute hepatitis B (AHB) and seven healthy liver donors (HC) were used as controls. Samples of liver and blood from both FHF patients and HC were obtained during transplantation procedures. Plasma levels of IL-1β, IL-4, IL-6, IL-8, IL-10, IFN-γ, TNF-α, MCP-1, RANTES and MIP-1α were quantified using a multiplex immunoassay. Cell characterization was carried out by flow cytometry. IFN-γ staining was performed on liver sections using immunofluorescence methods. An increase of peripheral frequency of natural killer (NK) cells expressing early activation markers (CD69, HLA-DR and CD38) and adhesion molecule CD44 was observed in FHF patients. Elevated frequency of T lymphocytes CD4(+) and CD8(+) expressing CD38 and adhesion molecules CD29 and CD44 was also observed in FHF. Additionally, an increase of natural killer T cells (NKT) was detected in FHF patients. High plasma cytokine levels were not statistically different between FHF and AHB patients. In comparison to ...Continue Reading

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References

Mentioned in this Paper

Flow Cytometry
Immunofluorescence Assay
Invariant Natural Killer T-Cells
CD69 wt Allele
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Recombinant Interleukin-10
Interleukin-1
Suppressor T-Lymphocytes, CD8-Positive
Hepatic
Monocyte Chemoattractant Protein-1

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