Activated lymphocytes increase expression of 5-lipoxygenase and its activating protein in THP-1 cells

The American Journal of Physiology
W L RingTimothy D Bigby

Abstract

The aim of this study was to investigate the regulation of the 5-lipoxygenase pathway of arachidonic acid metabolism by lymphocytes using the monocyte-like cell line, THP-1. When THP-1 cells were incubated over 4-7 days in 10% supernatant from lectin-activated human lymphocytes, their capacity to synthesize 5-lipoxygenase products was significantly increased. In contrast, the supernatant from nonactivated lymphocytes had no effect. The increase in capacity to synthesize 5-lipoxygenase products was mimicked by the addition of either granulocyte macrophage colony-stimulating factor (GM-CSF) or interleukin-3. These increases in synthetic capacity reflected increased enzymatic activity. Increased immunoreactive protein and mRNA for the enzymes 5-lipoxygenase and 5-lipoxygenase-activating protein were also found in cells conditioned with activated lymphocyte supernatants. Furthermore, the increase in mRNA for both enzymes was not blocked by cycloheximide, suggesting that the effect on steady-state mRNA levels does not require the synthesis of new protein. The increase in mRNA could be reproduced by GM-CSF. We conclude that lymphocytes can regulate the expression of 5-lipoxygenase in THP-1 cells over a period of days via the release ...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A SchönthalJ R Feramisco
Jan 1, 1992·American Journal of Respiratory Cell and Molecular Biology·N MeslierT D Bigby
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·M BrungsD Steinhilber
Mar 1, 1994·The Journal of Clinical Investigation·D A MunafoT D Bigby
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SteinhilberB Samuelsson

❮ Previous
Next ❯

Citations

Mar 6, 2004·Pharmacological Reviews·Charles BrinkTakehiko Yokomizo
Aug 7, 1999·Clinical Reviews in Allergy & Immunology·T D Bigby
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·O P Rådmark
Jan 16, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Saskia BuschDieter Steinhilber
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S J ChuW R Henderson
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kenneth J SerioTimothy D Bigby
Mar 14, 2021·Nature Communications·Alexandre F AissaElizaveta V Benevolenskaya
Aug 5, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Peters-Golden, T G Brock

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.