PMID: 9159648Feb 1, 1997Paper

Activated macrophage-mediated endogenous prostaglandin and nitric oxide-dependent relaxation of lymphatic smooth muscles

The Japanese Journal of Physiology
H Wang

Abstract

The effects of macrophages activated by bacterial lipopolysaccharides (LPS) on the mechanical activity of lymph vessels with or without the endothelium were investigated using conventional bioassay preparations. Rat peritoneal macrophages emigrated by an injection of thioglycollate were isolated and cultured for 12 h in RPMI 1,640 medium containing 10 micrograms/ml LPS. More than 97% of the cultured cells were stained with monoclonal antibody ED1 and demonstrated phagocytosis of acetylated low-density lipoprotein. The supernatant of the macrophages (M phi) suppressed significantly the basal tone of the lymphatic bioassay rings precontracted by 10(-8) M U46619. The M phi-induced vasodilation of the lymph nodes was significantly reduced by 12 h preincubation of the macrophages with 5 x 10(-5) M N omega-nitro-L-arginine methyl ester (L-NAME), 10(-5) M indomethacin, 10(-6) M dexamethasone, or 10(-5) M cycloheximide. Simultaneous preincubation of L-NAME and indomethacin caused a synergistic reduction of the M phi-induced vasodilation of the lymphatic bioassay rings. The superfusion of Krebs-bicarbonate solution containing 5 x 10(-5) M L-NAME, 5 x 10(-5) M aspirin, or the culture medium with no macrophages caused no significant effec...Continue Reading

Citations

Sep 13, 2013·Lymphatic Research and Biology·Shan Liao, Timothy P Padera
Dec 22, 1999·The Japanese Journal of Physiology·M MaedaM Nakai
Dec 1, 2009·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Pierre-Yves von der Weid, Mariappan Muthuchamy
Dec 5, 2012·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Cristina T KeslerTimothy P Padera
Jul 20, 2010·Alimentary Pharmacology & Therapeutics·P-Y von der Weid, K J Rainey
Oct 27, 2010·Annals of the New York Academy of Sciences·Pierre-Yves Von Der Weid, Sonia Rehal
Jan 27, 2005·Pharmacology & Therapeutics·Toshio OhhashiYoshiko Kawai
Oct 25, 2016·Experimental Cell Research·Pei ZhouDan Zhang
Oct 11, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Sanjukta ChakrabortyMariappan Muthuchamy
Apr 14, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·Y ShirasawaT Ohhashi
Mar 15, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·P S AndrewS Kaufman

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